Establishment and characterization of murine small cell lung carcinoma cell lines derived from HPV-16 E6/E7 transgenic mice

Autor: Massimo Riccio, Laura Carraresi, Rosanna Martinelli, Marcella Cintorino, Spartaco Santi, Maja Dembic, Mario Carmellini, Elisa Bigliardi, Alessandro Vannoni, Mara Rossini, Sergio Tripodi
Rok vydání: 2004
Předmět:
Cancer Research
Achaete-scute homologue 1
Lung Neoplasms
microtubule-associated protein 2
Cellular differentiation
Cell
chromogranin A
Neuroendocrine differentiation
Mice
Caveolin-1
Tumor Cells
Cultured
Carcinoma
Small Cell
Reverse Transcriptase Polymerase Chain Reaction
Chromogranin A
Cell Differentiation
Secretogranin 2
small cell lung carcinoma
DNA-Binding Proteins
medicine.anatomical_structure
Oncology
SEM
Syngenic
Small Cell Lung Carcinoma
secretogranin 2
caveolin-1
Transgene
Mice
Transgenic
Biology
Cytokeratin
confocal immunofluorescence
medicine
Confocal immunofluorescence
Animals
Humans
Small cell lung carcinoma
Microtubule-associated protein 2
Murine cell lines
Gene Expression Profiling
achaete-scute homologue 1
HPV-16 E6/E7 transgenic mice
murine cell lines
TEM
Oncogene Proteins
Viral
Molecular biology
Repressor Proteins
Achaete-scute homologue 1
Caveolin-1
Chromogranin A
Confocal immunofluorescence
HPV-16 E6/E7 transgenic mice
Microtubule-associated protein 2
Murine cell lines
Secretogranin 2
SEM
Small cell lung carcinoma
TEM
biology.protein
Biomarkers
Zdroj: Cancer letters
231:1 (2006): 65–73.
info:cnr-pdr/source/autori:L. CARRARESI, R. MARTINELLI, A. VANNONI, M. RICCIO, M. DEMBIC, S. TRIPODI, M. CINTORINO, S. SANTI, E. BIGLIARDI, M. CARMELLINI, M. ROSSINI,/titolo:Establishment and characterization of murine small cell lung carcinoma cell lines derived from HPV-16 E6%2FE7 transgenic mice,/doi:/rivista:Cancer letters (Print)/anno:2006/pagina_da:65/pagina_a:73/intervallo_pagine:65–73/volume:231:1
ISSN: 0304-3835
Popis: We have established two murine cell lines derived from Small Cell Lung Carcinomas (SCLCs) developed by HPV-E6/E7 transgenic mice. These cells named PPAP-9 and PPAP-10 were isolated from mice bearing tumors, 9 and 10 months old, respectively. The cells, 5 microm in diameter, express HPV oncoproteins and sustain tumor formation after subcutaneous injection in syngenic mice. A detailed analysis indicated the epithelial origin and the neuroendocrine differentiation of these cells. We showed by confocal immunofluorescence the expression of the epithelial marker cytokeratin 5, whose gene promoter was used to direct the expression of HPV E6/E. Cells express several neuroendocrine markers such as CGRP, MAP-2, Ash1, CgrA, Scg2. The neuroendocrine differentiation of these cells was further confirmed by electron microscopy demonstrating neuropeptides secreting granules in their cytoplasm. Furthermore, in agreement with the altered expression observed in the majority of human SCLC we showed in these cells the absence of both p53 and pRB and a dramatic reduction in the expression of Caveolin-1.
Databáze: OpenAIRE
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