Parameters of Hemostasis in Sheep Implanted with Composite Scaffold Settled by Stimulated Mesenchymal Stem Cells—Evaluation of the Animal Model
| Autor: | Marianna Gemra, Jakub Nicpoń, Piotr Skrzypczak, Janusz Bieżyński, Jarosław Król, Zdzisław Kiełbowicz, Przemysław Prządka, Dariusz Zalewski, Aleksandra Pliszczak-Król, Agnieszka Antończyk |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology
sheep Fibrinogen Article Andrology scaffold implantation medicine General Materials Science coagulation Fibroblast Clotting factor Microscopy QC120-168.85 Chemistry Antithrombin Mesenchymal stem cell QH201-278.5 Engineering (General). Civil engineering (General) TK1-9971 medicine.anatomical_structure Coagulation Descriptive and experimental mechanics Hemostasis hemostasis fibrinolysis Electrical engineering. Electronics. Nuclear engineering TA1-2040 Protein C medicine.drug circulatory and respiratory physiology |
| Zdroj: | Materials Volume 14 Issue 22 Materials, Vol 14, Iss 6934, p 6934 (2021) |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma14226934 |
| Popis: | Implantation of composite scaffolds could be potentially associated with the risk of hemostatic disturbances in a recipient. However, there is a lack of information on possible alterations in clotting mechanisms resulting from such a procedure. The aim of the present work was to investigate changes in hemostatic parameters in sheep implanted with a scaffold composed of poly(ε-caprolactone) and hydroxyapatite and tricalcium phosphate (9:4.5:4.5), settled previously with mesenchymal stem cells stimulated by fibroblast growth factor-2 and bone morphogenetic protein-2. Nine Merino sheep were examined for 7 days, and measurements of clotting times (PT, aPTT), activities of antithrombin, protein C and clotting factors II-XII, and concentrations of fibrinogen and D-dimer were carried out before and 1 h, 24 h, 3 days and 7 days after scaffold implantation. The introduction of scaffold initially resulted in a slowdown of the clotting processes (most evident 24 h after surgery) PT and aPTT increased to 14.8 s and 33.9 s, respectively. From the third day onwards, most of these alterations began to return to normal values. The concentration of fibrinogen rose throughout the observation period (up to 8.4 g/L), mirroring the ongoing inflammatory reaction. However, no signals of significant disturbances in hemostatic processes were detected in the sheep tested. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|