Peripheral blood monocytes from the decompensated liver cirrhosis could migrate into nude mouse liver with human hepatocyte-markers expression
| Autor: | Daiming Fan, Ying Han, Honghong Wang, Yuanlong He, Li Yan, Jingbo Wang, Jingmei Liu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Liver Cirrhosis
Pathology medicine.medical_specialty Cirrhosis Biophysics Mice Nude Biochemistry Peripheral blood mononuclear cell Monocytes Mice Nude mouse Western blot Cell Movement Albumins Granulocyte Colony-Stimulating Factor medicine Animals Humans Genes sry Organic Chemicals Molecular Biology DNA Primers Fluorescent Dyes Staining and Labeling medicine.diagnostic_test biology Reverse Transcriptase Polymerase Chain Reaction business.industry Stem Cells hemic and immune systems Cell Biology medicine.disease biology.organism_classification Immunohistochemistry Recombinant Proteins Granulocyte colony-stimulating factor Transplantation Liver Immunology Hepatocytes Female business Biomarkers Stem Cell Transplantation Adult stem cell |
| Zdroj: | Biochemical and Biophysical Research Communications. 371:635-638 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2008.04.058 |
| Popis: | Adult stem cells provide a promising alternative for the treatment of decompensated liver cirrhosis. Our previous study showed that peripheral blood monocytes (PBMCs) from decompensated liver cirrhosis could differentiate into hepatocyte-like cells in vitro. We here aimed to investigate the differential potential of the PBMCs under liver environment. PBMCs were isolated from one cirrhotic patient, who was mobilized by the recombinant human granulocyte colony stimulating factor for consecutive 3 days, and then PBMCs were transplanted into nude mice by tail vein after labeled with PKH26-GL. The location of the transplanted PBMCs was identified by PKH26-GL staining and PRINS for human SRY gene. The expressions of human hepatocyte-markers were detected by immunohistochemistry, RT-PCR, and Western blot analysis. Our results demonstrated that PBMCs from decompensated liver cirrhosis could migrate into the liver of nude mice with human hepatocyte-markers expression, indicating that autologous PBMCs transplantation might be one alternative therapy for the decompensated liver cirrhosis. |
| Databáze: | OpenAIRE |
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