Orexin A in the Rostrolateral Hypothalamic Area Induces Feeding by Modulating GABAergic Transmission

Autor: Donald C. Sweet, J.L. Beverly, Dolores Fernandez Doane, A. J. Thorpe, Catherine M. Kotz
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Male
medicine.medical_specialty
Baclofen
Time Factors
Lateral hypothalamus
Microdialysis
Biology
Bicuculline
gamma-Aminobutyric acid
Article
GABA Antagonists
Rats
Sprague-Dawley
chemistry.chemical_compound
Orexin-A
Eating
Internal medicine
mental disorders
medicine
Animals
Drug Interactions
Molecular Biology
GABA Agonists
gamma-Aminobutyric Acid
Analysis of Variance
Orexins
Behavior
Animal
Dose-Response Relationship
Drug
Muscimol
General Neuroscience
digestive
oral
and skin physiology
Neuropeptides
Intracellular Signaling Peptides and Proteins
GABA receptor antagonist
Orexin
Rats
Endocrinology
chemistry
nervous system
Hypothalamic Area
Lateral
Saclofen
GABAergic
Neurology (clinical)
psychological phenomena and processes
hormones
hormone substitutes
and hormone antagonists
Developmental Biology
medicine.drug
Popis: The neuromodulatory peptides orexin A and B are important central nervous system regulators of appetite. We previously identified the rostral lateral portion of the hypothalamus as an area important to orexin A feeding regulation. As γ-aminobutyric-acid (GABA) within the lateral hypothalamus also mediates feeding, we sought to determine the relationship between orexin and GABA signaling within this site. Adult male Sprague Dawley rats were implanted with cannulae directed to the rostral lateral hypothalamus and saclofen (GABA-B receptor antagonist), biccuculine (GABA-A receptor antagonist), or muscimol (GABA-A receptor agonist) were injected prior to orexin A. Both GABA antagonists failed to significantly affect orexin A-induced feeding, but muscimol significantly and dose dependently inhibited orexin A-induced feeding. Using in vivo microdialysis GABA release within this region significantly dropped during the first hour following orexin A administration, coinciding with orexin A induced feeding. Together, these data indicate that orexin A may influence food intake by decreasing GABAergic tone within the rostral lateral hypothalamus.
Databáze: OpenAIRE
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