Enhancement of the immunogenicity of a synthetic peptide bearing a VP3 epitope of hepatitis A virus
| Autor: | Monica Garcia, Rosa M. Pintó, Juan F. González-Dankaart, Albert Bosch, Gloria Sánchez, Isabel Haro, Susana Guix, M. José Gómara |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
T-epitope
B-epitope viruses Biophysics Epitopes T-Lymphocyte Enzyme-Linked Immunosorbent Assay Peptide Antibodies Viral medicine.disease_cause Biochemistry Epitope Cell Line Mice Capsid Immune system Antigen Neutralization Tests Structural Biology Genetics medicine Animals Hepatovirus Bovine serum albumin Molecular Biology Hepatitis B virus chemistry.chemical_classification Drug Carriers biology Immunogenicity virus diseases Cell Biology biochemical phenomena metabolism and nutrition Virology Molecular biology Peptide Fragments Liposome Synthetic peptide chemistry Liposomes biology.protein Epitopes B-Lymphocyte Capsid Proteins Female Hepatitis A virus Antibody |
| Zdroj: | FEBS Letters. 438:106-110 |
| ISSN: | 0014-5793 |
| Popis: | The immune responses elicited in mice by different forms of the VP3(110–121) B-epitope of the hepatitis A virus (HAV) were studied. Different forms of incorporation in liposomes were tested, encapsulation, rather than surface exposure, being the best antigenic preparation. Three larger peptides of the VP3 epitope, two of them containing a hepatitis B virus T-epitope, and a third containing a putative T-epitope of HAV (VP3(102–121)) were assayed. While this latter T-epitope induced an enhancement of the response against the VP3 B-epitope, the artificially coupled T-epitopes failed to induce a significant increase. The administration of two multiple antigenic peptide (MAP) constructs, the first containing the VP3(110–121) and VP1(11–25) HAV sequences and the second only the VP1(11–25) sequence, also suggested the presence of a T-epitope, since the response against the VP1 peptide was increased in the first construct. |
| Databáze: | OpenAIRE |
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