Identification of distinct seminal plasma cytokine profiles associated with male age and lifestyle characteristics in unexplained recurrent pregnancy loss
| Autor: | S. le Cessie, N. A. du Fossé, E. van Beelen, M.L.P. van der Hoorn, J.M.M. van Lith, Michael Eikmans, Tess Meuleman, Eileen E L O Lashley |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Abortion Habitual Chemokine medicine.medical_treatment Immunology Physiology Cytokine Expression Profile Immune tolerance Cluster analysis Pregnancy Semen medicine Humans Immunology and Allergy Seminal plasma Fetus biology business.industry Other Research Radboud Institute for Health Sciences [Radboudumc 0] Age Factors Obstetrics and Gynecology Prognosis medicine.disease Lifestyle Healthy Volunteers Semen Analysis Identified patient Recurrent pregnancy loss Cytokine Reproductive Medicine Case-Control Studies biology.protein Cytokines Female business Body mass index Biomarkers |
| Zdroj: | Journal of Reproductive Immunology, 147 Journal of Reproductive Immunology, 147. ELSEVIER IRELAND LTD |
| ISSN: | 0165-0378 |
| Popis: | Contains fulltext : 244625.pdf (Publisher’s version ) (Open Access) BACKGROUND: Seminal plasma contains a wide range of cytokines, chemokines and growth factors. Part of these signalling molecules assist in inducing a state of active maternal immune tolerance towards the fetus. Disbalances in seminal plasma content may contribute to pregnancy loss. This study investigated cytokine expression profiles in seminal plasma of male partners of couples with unexplained recurrent pregnancy loss (RPL) and the association with clinical and lifestyle characteristics, including smoking, alcohol consumption and body mass index (BMI). METHODS: In the seminal plasma of 52 men who visited a specialised RPL clinic the levels of 25 pre-selected cytokines, chemokines and growth factors were measured by Bio-Plex assay or ELISA. Two-way hierarchical cluster analysis was performed. Identified patient clusters were compared on clinical and lifestyle characteristics. RESULTS: Two distinct cytokine expression profiles in the seminal plasma were revealed by cluster analysis. Patient cluster I showed relatively higher levels of pro-inflammatory cytokines, including IL-1α, IL-1β, IL-6, IL-8, IL-12, IL-18 and TNF-α, compared to Patient cluster II. Men belonging to Patient cluster I were significantly older and had significantly more lifestyle risk factors compared to men in Patient cluster II. CONCLUSION: Cluster analysis suggested the existence of a less favourable pro-inflammatory cytokine expression profile, being present in part of men affected by RPL and associated with advanced male age and lifestyle risk factors. These findings may serve as a starting point for further research into underlying mechanisms and ultimately lead to novel diagnostic and therapeutic approaches for couples with RPL. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect