| Autor: |
Yan Zhou, Zhi Liu, Shubing Tang, Nan Yue, Shuhua Tang, Zhe Zhang, Hongyun Hao, Yuan Weiming, Baoqin Xuan, Zhikang Qian, Xiaoqi Yang, Deng Pan, Tian Han, Wei Zhou, Zewei Wang, Sirwan S. Sleman |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
ACS applied bio materials. 3(7) |
| ISSN: |
2576-6422 |
| Popis: |
Antibodies are fundamental tools for basic science; however, high-quality antibodies suitable for multiple experimental applications are often inaccessible to research laboratories. To this end, a modular and low-cost pipeline for small-scale antibody customization is developed. First, soluble antigens are designed according to the secondary structure of a desired protein. Then, the antigens are efficiently displayed on a modular nanoplatform by intein-mediated trans-splicing (TS) that enables elicitation of high titers of protein-specific antibodies. After that, target antibodies are obtained by a modular HaloLink resin platform with antigens as the ligand that is devised by intein-mediated TS. Finally, purified antibodies show excellent properties in immunofluorescence, immunoprecipitation, and western blotting assays. Overall, these results suggest that the proposed pipeline is amenable to the generation of high-quality, research-grade antibodies and to aid in protein functional studies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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