Engineering of a vascularized scaffold for artificial tissue and organ generation
| Autor: | Heike Mertsching, Wolfram H. Knapp, Johanna Schanz, Michael Hofmann, Thorsten Walles |
|---|---|
| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
CD31
Scaffold Pathology medicine.medical_specialty Time Factors Swine Biophysics Bone Marrow Cells Bioengineering Stain Regenerative medicine Biomaterials Tissue engineering Antigens CD Fluorodeoxyglucose F18 Cell Adhesion Animals Regeneration Medicine Endothelium Progenitor cell Bioartificial Organs Tissue Engineering medicine.diagnostic_test business.industry Stem Cells Cell Differentiation DNA Cadherins Immunohistochemistry Platelet Endothelial Cell Adhesion Molecule-1 Mechanics of Materials Positron emission tomography Positron-Emission Tomography Ceramics and Composites Endothelium Vascular business Artificial tissue |
| Popis: | Tissue engineering is an emerging field in regenerative medicine to overcome the problem of end-stage organ failure. However, complex tissues and organs need a vascular supply to guaranty graft survival and render bioartificial organ function. Here we developed methods to decellularize porcine small bowl segments and repopulate the remaining venous and arterial tubular structures within these matrices with allogeneic porcine endothelial progenitor cells. Cellular adherence and vitality was characterized by quantitative 2-[18F]-fluoro-2′-desoxy-glucose (FDG) positron emission tomography (PET) and subsequent immunohistological work up. The generated matrices showed insulin-dependent FDG uptake predominantly in the region of the former vascular structures. Stain for vitality and the specific endothelial markers CD31, VE-Cadherin and Flk-1 matched this functional finding. Providing evidence for vitality up to 3 weeks post reconstitution and typical endothelial differentiation, these results indicate that our generated matrix allows the generation of complex bioartificial tissues and organs for experimental and future clinical application. |
| Databáze: | OpenAIRE |
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