Changes in progesterone and oestrogen receptor mRNA and protein and oxytocin receptors in endometrium of ewes after intrauterine injection of ovine trophoblast interferon
| Autor: | T F Ogle, Mark A. Mirando, Y Zhou, R J Moffatt, Jacob P. Harney, Troy L. Ott, Fuller W. Bazer |
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| Rok vydání: | 1993 |
| Předmět: |
Fetal Proteins
Receptors Vasopressin medicine.medical_specialty Oxytocin receptor binding Biology Endometrium Endocrinology Pregnancy Internal medicine medicine Animals Conceptus Receptor Molecular Biology Estrous cycle Analysis of Variance Sheep Trophoblast Oxytocin receptor Trophoblasts medicine.anatomical_structure Receptors Estrogen Oxytocin Receptors Oxytocin Interferon Type I Female Receptors Progesterone medicine.drug |
| Zdroj: | Journal of Molecular Endocrinology. 10:185-192 |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1677/jme.0.0100185 |
| Popis: | This study determined whether twice-daily intrauterine injections of ovine conceptus secretory proteins (oCSP) containing type-I trophoblast interferon (25 μg/uterine horn) from day 11 to day 15 post-oestrus (oestrus = day 0) could alter the binding capacities of endometrial receptors for oxytocin, progesterone and oestrogen in cyclic ewes when compared with control ewes receiving serum protein (SP) injections. Injections of oCSP on days 11–15 post-oestrus decreased concentrations of oestrogen receptors (PPPPPm oxytocin in vitro was highly correlated with the concentration (r≥0·93, PKd (r= –0·91, Pr≤0·83, PKd (r≤ –0·40, P>0·40) of oxytocin receptors in oCSP-infused ewes. This indicates that oCSP disrupted the relationship between oxytocin receptor binding and oxytocin-induced activation of its second messenger system. These results indicate that antiluteolytic type-I trophoblast interferon may prevent oxytocininduced luteolytic pulsatile secretion of prostaglandin F2α during maternal recognition of pregnancy in sheep, by reducing the synthesis and affinity of endometrial oxytocin receptors. Inhibition of other components of the oxytocin receptor—phospholipase C system by ovine trophoblast interferon may also be involved in reducing endometrial responsiveness to oxytocin. Ovine trophoblast interferon may inhibit the synthesis of endometrial oestrogen receptors to inhibit responsiveness to oxytocin during early pregnancy in ewes. |
| Databáze: | OpenAIRE |
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