Significance of expression of pyrimidine metabolizing genes in colon cancer
Autor: | Thanaa F Moghazy, R. S. Hafez, Heba Morsi, Mohamed Shamesya, Mohamed Samir, Ragaa A Ramadan |
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Rok vydání: | 2019 |
Předmět: |
Colorectal cancer
business.industry Gastroenterology Thymidylate Synthase medicine.disease_cause medicine.disease Metastasis 03 medical and health sciences 0302 clinical medicine Pyrimidines Tumor progression 030220 oncology & carcinogenesis Gene expression Colonic Neoplasms medicine Dihydropyrimidine dehydrogenase Cancer research Humans 030211 gastroenterology & hepatology DPYD Fluorouracil Thymidine phosphorylase Carcinogenesis business Dihydrouracil Dehydrogenase (NADP) |
Zdroj: | Arab journal of gastroenterology : the official publication of the Pan-Arab Association of Gastroenterology. 21(3) |
ISSN: | 2090-2387 |
Popis: | Background and study aims The reprogramming of metabolic pathways in tumour cells is a crucial step to meet the increased requirements for their own growth. This process occurs through alterations in gene expression, polymorphisms, and epigenetic dysregulation of a number of metabolic genes. Several metabolic enzymatic pathways such as pyrimidine-metabolizing enzymes have been implicated in tumorigenesis and tumor progression. Patients and methods We measured the relative expression levels of three pyrimidine-metabolizing genes—thymidylate synthase (TYMS), thymidine phosphorylase (TYMP), and dihydropyrimidine dehydrogenase (DPYD)—in tumor tissue and adjacent normal-appearing mucosa in 50 colon cancer (CC) patients using real-time reverse-transcription polymerase chain reaction. Gene expression was also studied in relation to demographic and pathological criteria. Results The gene expression levels of both TYMS and TYMP were significantly higher in tumor tissue than normal adjacent tissue. Further, they showed an agreeable level of diagnostic performance as a means to discriminate between normal and tumor tissue; TYMS had high specificity (94%) but moderate sensitivity (60%), while TYPM showed average sensitivity (70%) and specificity (76%). Although DPYD expression was lower in tumor tissue than paracancerous tissue, this level did not reach the statistical significance. TYMS expression was significantly higher in moderately and poorly differentiated tumors than in well-differentiated ones. There was no significant association between gene expression and the remaining clinicopathological criteria (e.g., age, sex, tumor location, and metastasis). We found a positive correlation between the gene expression levels of TYMS and DPYD. Conclusion TYMS and TYMP messenger RNA levels seem to be plausible indicators in the diagnosis of CC, although further studies are warranted for validation. |
Databáze: | OpenAIRE |
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