Detection of Specific Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus Nucleocapsid Protein for Serodiagnosis of SARS Coronavirus Pneumonia
| Autor: | Kwok-Yung Yuen, J. S. Malik Peiris, Ami M. Y. Fung, Susanna K. P. Lau, Beatrice H.L. Wong, Victoria K. P. Tam, Hoi Wah Tsoi, Kwok-Hung Chan, Patrick C. Y. Woo |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Immunoglobulin A viruses Enzyme-Linked Immunosorbent Assay Antibodies Viral Severe Acute Respiratory Syndrome medicine.disease_cause Sensitivity and Specificity SARS Virus - immunology Immunoglobulin G Serology Nucleocapsid Proteins - immunology Antibody Specificity Virology medicine Coronavirus Nucleocapsid Proteins Humans Serologic Tests skin and connective tissue diseases Antigens Viral Coronavirus biology fungi Antibodies Viral - blood Nucleocapsid Proteins medicine.disease respiratory tract diseases body regions Pneumonia Immunoglobulin M Severe acute respiratory syndrome-related coronavirus Case-Control Studies Immunology biology.protein Severe Acute Respiratory Syndrome - diagnosis - immunology Severe acute respiratory syndrome Antibody |
| Zdroj: | Journal of Clinical Microbiology |
| ISSN: | 0095-1137 |
| DOI: | 10.1128/jcm.42.5.2306-2309.2004 |
| Popis: | We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 94.3, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV. published_or_final_version |
| Databáze: | OpenAIRE |
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