Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: prevalence and virulence properties
| Autor: | Ángel Adrián Cataldi, Germán Chillemi, Isabel Chinen, H. Ricardo Rodríguez, Marta Rivas, Elizabeth Miliwebsky, Beatriz E. C. Guth, Andrea Gioffré, Marcelo O. Masana, Ariela Baschkier, L. Meichtri |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Serotype Argentina Cattle Diseases Virulence Biology medicine.disease_cause Polymerase Chain Reaction Microbiology Shiga Toxin law.invention Feces law Genotype Escherichia coli Prevalence medicine Animals Serotyping Escherichia coli Infections Polymerase chain reaction Disease Reservoirs Rectum O Antigens Shiga toxin General Medicine biology.organism_classification Enterobacteriaceae Diarrhea biology.protein Cattle medicine.symptom Abattoirs Food Science |
| Zdroj: | International Journal of Food Microbiology. 96:189-198 |
| ISSN: | 0168-1605 |
| DOI: | 10.1016/j.ijfoodmicro.2004.03.018 |
| Popis: | Between July 1999 and December 2000, the prevalence of Shiga toxin-producing Escherichia coli (STEC) was established in 200 Argentine healthy young beef steers (14–16 months old) grown under local production systems with a feed grain period of 3–4 months, and the STEC strains isolated were examined in regard to their phenotypic and genotypic characteristics. Stool samples ( n =70) and rectal swabs ( n =130) were taken at the slaughterhouse level. By polymerase chain reaction (PCR), Shiga toxin ( stx ) gene sequences were detected in 69% of the samples. Eighty-six STEC strains were isolated from 39% of the animals. Serogroups identified, in order of frequency, were: O8 (16 strains), O113 (14), O103 (5), O91 (4), O171 (3), O174 (3), O25 (2), O112 (2), O145 (2), O2, O11, O104, O121, O128, O143, O146, O157. The most frequent serotype isolated was O8:H19 (12.9%). A total of 17 serotypes, including E. coli O157:H7 found in one animal (0.5%), have been previously associated with hemolytic uremic syndrome (HUS), bloody and non-bloody diarrhea in different countries, including Argentina. The prevalent genotype isolated was stx 2 (51 of 86, 59.3%). Subtyping of stx 2 variants showed the prevalence of stx 2vh-b (25.6%) and stx 2vh-a types (24.4%), and revealed the presence of an atypical stx 2-v. Only 7.0% of STEC strains carried eae , and 33.7% harbored EHEC- hly A gene. The full virulent genotype ( stx / eae /EHEC- hly A) was found to be present in 4 of the 86 (4.7%) STEC strains isolated. This research indicates that young steers from the main beef-producing area of Argentina are an important reservoir of STEC strains; however, its importance as agents of human diseases in our country has still to be established. |
| Databáze: | OpenAIRE |
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