Cumulus cells of euploid versus whole chromosome 21 aneuploid embryos reveal differentially expressed genes
| Autor: | Shiny Titus, Rolando Garcia-Milian, Ashley W. Tiegs, Sameet Mehta, Richard T. Scott, Emre Seli |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
endocrine system Monosomy Serum Response Factor Chromosomes Human Pair 21 Dishevelled Proteins Biology Proof of Concept Study Transcriptome Andrology Pregnancy Gene expression medicine Humans Gene Cumulus Cells Obstetrics and Gynecology Embryo medicine.disease Embryo Mammalian Reproductive Medicine embryonic structures Oocytes Female Down Syndrome Trisomy Chromosome 21 Embryo quality Biomarkers Developmental Biology Cysteine-Rich Protein 61 |
| Zdroj: | Reproductive biomedicine online. 43(4) |
| ISSN: | 1472-6491 |
| Popis: | Research question Can cumulus cells be used as a non-invasive target for the study of determinants of preimplantation embryo quality? Design Cumulus cells were collected from monosomy 21, trisomy 21 and euploid embryos and subjected to RNA sequencing analysis and real-time polymerase chain reaction assays. The differential gene expression was analysed for different comparisons. Results A total of 3122 genes in monosomy 21 cumulus cells and 19 genes in trisomy 21 cumulus cells were differentially expressed compared with euploid cumulus cells. Thirteen of these genes were differentially expressed in both monosomy and trisomy 21, compared with euploid, including disheveled segment polarity protein 2 (DVL2), cellular communication network factor 1 (CCN1/CYR61) and serum response factor (SRF), which have been previously implicated in embryo developmental competence. In addition, ingenuity pathway analysis revealed cell–cell contact function to be affected in both monosomy and trisomy 21 cumulus cells. Conclusions These findings support the use of cumulus cell gene expression analysis for the development of biomarkers evaluating oocyte quality for patients undergoing fertility preservation of oocytes. |
| Databáze: | OpenAIRE |
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