The AAA+ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization
| Autor: | Jiuya He, John E. Walker, Alan J. Robinson, Ian J. Holt, Johannes N. Spelbrink, Michael E. Harbour, Chih-Chieh Mao, Aurelio Reyes, Andrew B. Clippingdale, Stefanie Reichelt, Miriam Di Re, Ian M. Fearnley, Caroline Granycome, Hiroshi Sembongi |
|---|---|
| Přispěvatelé: | Reyes Tellez, Aurelio [0000-0003-2876-2202], Robinson, Alan [0000-0001-9943-0059], Walker, John [0000-0001-7929-2162], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Mitochondrial DNA
Submitochondrial Particles DNA Single-Stranded Electrophoretic Mobility Shift Assay Mitochondria Liver Biology MT-RNR1 Binding Competitive DNA Mitochondrial Mitochondrial Proteins 03 medical and health sciences 0302 clinical medicine Adenosine Triphosphate Report Cell Line Tumor Animals Humans Electrophoresis Gel Two-Dimensional RNA Small Interfering Mitochondrial nucleoid organization Research Articles 030304 developmental biology Mitochondrial nucleoid HSPA9 Adenosine Triphosphatases 0303 health sciences Binding Sites DNA Superhelical Membrane Proteins Cell Biology Mitochondrial carrier Peptide Fragments 3. Good health Rats DNA-Binding Proteins Nucleoproteins Biochemistry ATPases Associated with Diverse Cellular Activities Nucleic Acid Conformation Mitochondrial fission ATP–ADP translocase 030217 neurology & neurosurgery Plasmids Protein Binding |
| Zdroj: | The Journal of Cell Biology |
| Popis: | Many copies of mammalian mitochondrial DNA contain a short triple-stranded region, or displacement loop (D-loop), in the major noncoding region. In the 35 years since their discovery, no function has been assigned to mitochondrial D-loops. We purified mitochondrial nucleoprotein complexes from rat liver and identified a previously uncharacterized protein, ATAD3p. Localization studies suggested that human ATAD3 is a component of many, but not all, mitochondrial nucleoids. Gene silencing of ATAD3 by RNA interference altered the structure of mitochondrial nucleoids and led to the dissociation of mitochondrial DNA fragments held together by protein, specifically, ones containing the D-loop region. In vitro, a recombinant fragment of ATAD3p bound to supercoiled DNA molecules that contained a synthetic D-loop, with a marked preference over partially relaxed molecules with a D-loop or supercoiled DNA circles. These results suggest that mitochondrial D-loops serve to recruit ATAD3p for the purpose of forming or segregating mitochondrial nucleoids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|