Supplementary Figure 2 from Maximizing the Efficacy of MAPK-Targeted Treatment in PTENLOF/BRAFMUT Melanoma through PI3K and IGF1R Inhibition

Autor: Saskia M. Brachmann, Michael N. Hall, Francesco Hofmann, William R. Sellers, Sauveur-Michel Maira, Keith Calkins, Mitko Ristov, Daniel Guthy, Mark Stump, David Ruddy, Eric Billy, Malika Kazic-Legueux, Grégory Marszalek, Hélène Erasimus, Janina Juengert, Hans Voshol, Thomas Ferrat, Christian Schnell, Sandra Mollé, Audrey Kauffmann, Barbara Herkert
Rok vydání: 2023
DOI: 10.1158/0008-5472.22408862
Popis: PI3Kβ inhibition induces p85/IRS2 interaction in a time-dependent manner in the PTENLOF/BRAFMUT melanoma cell line RVH-421 that is mediated by negative feedback signaling. A) Co-immunoprecipitation experiments were carried out using cell lysates of RVH-421 treated with the PI3Kbi for the indicated time and blotted for p85, and pTyr (IRS2). B)-D) Input lysates of the immunoprecipitation experiments shown in Figure 4 were immunoblotted with the indicated phospho-specific or total target protein antibodies. PI3Kβi=rac-KIN-193, mTORi=AZD8055, mTORCi=RAD001, PDK1i=GSK-2334470, AKTi=MK2206
Databáze: OpenAIRE