Estrogen-sensitive progestin-binding sites in the female rat brain and pituitary
| Autor: | Martine Moguilewsky, Jean-Pierre Raynaud |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Receptor complex
medicine.medical_specialty Norpregnadienes medicine.drug_class Hypothalamus Moxestrol Biology Promegestone chemistry.chemical_compound Estradiol Congeners Internal medicine Centrifugation Density Gradient medicine Animals Castration Binding site Receptor Molecular Biology Cerebral Cortex Binding Sites urogenital system General Neuroscience Uterus Brain Adrenalectomy Rats Dissociation constant Endocrinology chemistry Estrogen Pituitary Gland Female Neurology (clinical) Receptors Progesterone hormones hormone substitutes and hormone antagonists Developmental Biology |
| Zdroj: | Brain Research. 164:165-175 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(79)90013-1 |
| Popis: | The following properties of the cytoplasmic progestin receptor were studied in the hypothalamus, cortex, pituitary and uterus of the estrogen-primed castrated adult female rat using the highly potent progestin R 5020 (promegestone). (a) Sedimentation pattern. In sucrose density gradients, the R 5020-progestin receptor complex sedimented with a coefficient of about 6 to 7S. (b) Binding parameters. R 5020 bound to the progestin receptor with an intrinsic dissociation constant of about 10(-9) M as measured by a Dextran-coated charcoal (DCC) technique. The number of binding sites, however, differed widely. (c) Specificity. Only progestins competed for [3H]R 5020 binding. (d) Estrogen-dependency. In both immature and castrated adult rats, estrogen administration increased the number of R 5020-specific binding sites, assayed in vitro by a DCC technique, in the uterus, pituitary and hypothalamus, but not in the amygdala, hippocampus nor in the cortex. The increase was maximum between 40 and 48 h after priming with the potent estrogen, moxestrol, and could not be induced by androgens nor by progestins. |
| Databáze: | OpenAIRE |
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