Nucleic Acid Nanocapsules for Enzyme-Triggered Drug Release
| Autor: | Joseph K. Awino, Joshua J. Santiana, Saketh Gudipati, Alyssa K. Hartmann, Dominic F. Cairns-Gibson, Jessica L. Rouge, Nicole Gomez |
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| Rok vydání: | 2017 |
| Předmět: |
Cell Survival
Surface Properties Deoxyribozyme 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Catalysis Nanocapsules Colloid and Surface Chemistry Humans Organic chemistry Particle Size Chemistry Esterases DNA DNA Catalytic General Chemistry 021001 nanoscience & nanotechnology Small molecule Combinatorial chemistry 0104 chemical sciences Drug Liberation Click chemistry Nucleic acid Surface modification Particle Particle size 0210 nano-technology Hydrophobic and Hydrophilic Interactions HeLa Cells |
| Zdroj: | Journal of the American Chemical Society. 139:6278-6281 |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | Herein we describe a nucleic acid functionalized nanocapsule in which nucleic acid ligands are assembled and disassembled in the presence of enzymes. The particles are fully degradable in response to esterases due to an embedded ester cross-linker in the particle's core. During synthesis the nanocapsules can be loaded with hydrophobic small molecules and post self-assembly undergo covalent cross-linking using copper catalyzed click chemistry. They can then be functionalized with thiolated DNA through stepwise thiolyne chemistry using UV light irradiation. Additionally, the capsule is compatible with enzyme mediated functionalization of a therapeutic mRNA-cleaving DNAzyme at the particle's surface. The resulting particle is highly stable, monodisperse in size, and maximizes the therapeutic potential of both the particles interior and exterior. |
| Databáze: | OpenAIRE |
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