Establishment of the cells useful for murine interleukin-18 bioassay by introducing murine interleukin-18 receptor cDNA into human myelomonocytic KG-1 cells
| Autor: | Hiroshi Yamauchi, Takanori Okura, Shimpei Ushio, Masashi Kurimoto, Tsutomu Mori, Yoshiyuki Nukada, Hakuo Ikegami, Tsunetaka Ohta, Mutsuko Taniguchi, Katsue Nagaoka |
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| Rok vydání: | 1998 |
| Předmět: |
Lipopolysaccharides
DNA Complementary medicine.medical_treatment Recombinant Fusion Proteins Immunology Genetic Vectors Biology Monocytes Interferon-gamma Mice medicine Tumor Cells Cultured Immunology and Allergy Animals Humans Interferon gamma Propionibacterium acnes Receptor Receptors Interleukin-18 Genetic transfer Interleukin-18 Transfection Receptors Interleukin Molecular biology Endotoxemia Interleukin-18 receptor Mice Inbred C57BL Cytokine Cell culture Interleukin 18 Biological Assay Interleukin-18 Receptor alpha Subunit medicine.drug Protein Binding Signal Transduction |
| Zdroj: | Journal of immunological methods. 217(1-2) |
| ISSN: | 0022-1759 |
| Popis: | We genetically engineered human myelomonocytic KG-I cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which were capable of responding to MuIL-18. These cells expressed larger number of MuIL-18R (13,000 sites/cell) than intrinsic human IL-18 receptor (HuIL-18R) (2,500 sites/cell). And the cells responded to MuIL-18 as well as to HuIL-18 in a dose-dependent manner, and produced large amounts of interferon-gamma (IFN-gamma). We could estimate the amount of murine IL-18 based on the amounts of IFN-gamma produced by these cells. The stoichiometry was observed up to 150 ng/ml of MuIL-18. By using these cells, a large amount of MuIL-18 (448 +/- 89.2 ng/ml) was detected in sera of Propionibacterium acnes (P. acnes)/lipopolysaccharide (LPS)-treated endotoxic mice (the same conditions in which IL-18 was first identified). These cells provide us with a useful tool for determining the bioactivity of MuIL-18. |
| Databáze: | OpenAIRE |
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