Translocation of protein kinase C in porcine thyroid cells following exposure to thyrotropin
| Autor: | Jody Ginsberg, Janice E. Parente, Patricia G. Murray, Kenneth Wong |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Phorbol ester
endocrine system medicine.medical_specialty endocrine system diseases Neutrophils Swine Thyroid Gland Biophysics Thyrotropin Chromosomal translocation In Vitro Techniques Biology Biochemistry Cytosol Protein kinase C Structural Biology Internal medicine Genetics medicine Animals Humans Thyroid cells Molecular Biology chemistry.chemical_classification (Thyroid cell) Cell Membrane Thyroid Cell Biology In vitro Enzyme Activation Enzyme medicine.anatomical_structure Endocrinology chemistry Tetradecanoylphorbol Acetate Thyroid function hormones hormone substitutes and hormone antagonists |
| Zdroj: | FEBS Letters. 226:223-226 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(88)81427-3 |
| Popis: | We have previously shown that protein kinase C activators modulate differentiated thyroid function in vitro; however, how protein kinase C may be activated physiologically is unknown. The present studies were undertaken in order to determine whether TSH could activate protein kinase C in vitro. Following exposure of porcine thyroid cells to TSH, translocation of protein kinase C from the cytosol to its membrane-bound form was observed. Maximal translocation occurred at the lowest TSH concentration able to trigger this response (10 mU/ml) but persisted at higher concentrations (20–100 mU/ml). Time-course studies revealed that translocation of protein kinase C was seen only after 40 min. TSH could also produce a similar translocation in human neutrophils (known to have TSH receptors). In thyroid cells pre-treated with TSH, modulation of phorbol-mediated protein kinase C translocation was noted. These results indicate that TSH causes the translocation of protein kinase C in porcine thyroid cells (and possibly other TSH receptor-containing cells) and therefore may regulate the action of protein kinase C on differentiated thyroid function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text