ω-3 fatty acids attenuate mucosal inflammation in premature rat pups

Autor: Kyoichi Oshida, Hirohisa Izumi, Kyo Okada, Eisuke Inage, Yoshikazu Ohtsuka, Yoko Yamakawa, Toshiaki Shimizu, Satoru Nagata, Tohru Fujii, Tamaki Ikuse, Yuichiro Yamashiro, Yosuke Baba
Rok vydání: 2011
Předmět:
medicine.medical_specialty
Docosahexaenoic Acids
Metabolite
Anti-Inflammatory Agents
Drug Evaluation
Preclinical
Peroxisome proliferator-activated receptor
Inflammation
Rats
Sprague-Dawley
Random Allocation
chemistry.chemical_compound
Dietary Fats
Unsaturated
Enterocolitis
Necrotizing
Ileum
Pregnancy
Internal medicine
medicine
Animals
Intestinal Mucosa
Maternal-Fetal Exchange
Enterocolitis
chemistry.chemical_classification
business.industry
Fatty Acids
NF-kappa B
General Medicine
medicine.disease
Eicosapentaenoic acid
Rats
Soybean Oil
Specific Pathogen-Free Organisms
PPAR gamma
Disease Models
Animal
Endocrinology
Animals
Newborn
Eicosapentaenoic Acid
Gene Expression Regulation
Eicosanoid
chemistry
Docosahexaenoic acid
Models
Animal
Receptors
Prostaglandin E
EP3 Subtype
Pediatrics
Perinatology and Child Health
Necrotizing enterocolitis
Female
Infant Food
lipids (amino acids
peptides
and proteins)
Surgery
medicine.symptom
business
Zdroj: Journal of Pediatric Surgery. 46:489-495
ISSN: 0022-3468
DOI: 10.1016/j.jpedsurg.2010.07.032
Popis: Necrotizing enterocolitis (NEC) is a devastating intestinal disease of premature infants. Although ω-3 fatty acids are known to have antiinflammatory effects, their effect against NEC remains unclear.Mother rats fed a soybean-based, docosahexaenoic acid (DHA)- or eicosapentaenoic acid (EPA)-enriched diet from days 7 to 20 of gestation were examined. On day 20, the rat pups were delivered by abdominal incision, their intestines were removed, and messenger RNA was extracted. A rat NEC model was used to confirm the effects of ω-3 fatty acids on the inflamed intestine (n = 20-28). The expression of inflammatory molecules was analyzed by real-time polymerase chain reaction (n = 11-14).The concentrations of DHA and EPA in the intestine were significantly increased in the DHA and EPA groups (P.01). The expression of the antiinflammatory prostaglandin E2 receptor EP3 was increased in the DHA (P.05) and EPA groups (P.01). In the NEC model, the reduced incidence of colitis was confirmed in the DHA and EPA groups. The expression of peroxisome proliferator-activated receptor γ was increased (P.05), and the inhibitor of nuclear factor-κB α/β decreased in both the DHA (P.01) and EPA groups (P.05).Our findings indicate that ω-3 fatty acids are beneficial for protecting the premature intestine from inflammation by regulating eicosanoid- and nuclear factor-κB-related metabolite expression.
Databáze: OpenAIRE
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