In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase
| Autor: | Scott A. Jones, Jianming Hu, Daniel N. Clark |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Hepatitis B virus Transcription Genetic Hepatitis B virus DNA polymerase Gene Expression Gene Products pol RNA-dependent RNA polymerase Priming (immunology) In Vitro Techniques Transfection Virus Replication Article Cell Line 03 medical and health sciences chemistry.chemical_compound Humans DNA Cleavage Polymerase DNA synthesis biology Phosphoric Diester Hydrolases Nuclear Proteins RNA Reverse Transcription Molecular biology Recombinant Proteins Reverse transcriptase DNA-Binding Proteins Enzyme Activation 030104 developmental biology chemistry Multiprotein Complexes biology.protein RNA Viral DNA Plasmids Protein Binding Transcription Factors |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493966981 Methods Mol Biol |
| DOI: | 10.1007/978-1-4939-6700-1_13 |
| Popis: | The hepatitis B virus (HBV) polymerase synthesizes the viral DNA genome from the pre-genomic RNA (pgRNA) template through reverse transcription. Initiation of viral DNA synthesis is accomplished via a novel protein priming mechanism, so named because the polymerase itself acts as a primer, whereby the initiating nucleotide becomes covalently linked to a tyrosine residue on the viral polymerase. Protein priming, in turn, depends on specific recognition of the packaging signal on pgRNA called epsilon. These early events in viral DNA synthesis can now be dissected in vitro as described here. The polymerase is expressed in mammalian cells and purified by immunoprecipitation. The purified protein is associated with host cell factors, is enzymatically active, and its priming activity is epsilon dependent. A minimal epsilon RNA construct from pgRNA is co-expressed with the polymerase in cells. This RNA binds to and co-immunoprecipitates with the polymerase. Modifications can be made to either the epsilon RNA or the polymerase protein by manipulating the expression plasmids. Also, the priming reaction itself can be modified to assay for the initiation or subsequent DNA synthesis during protein priming, the susceptibility of the polymerase to chemical inhibitors, and the precise identification of the DNA products upon their release from the polymerase. The identity of associated host factors can also be evaluated. This protocol closely mirrors our current understanding of the RNA binding and protein priming steps of the HBV replication cycle, and it is amenable to modification. It should therefore facilitate both basic research and drug discovery. |
| Databáze: | OpenAIRE |
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