Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis
| Autor: | Yong‑Qi Bai, Chun‑Yan Liu, Qu‑Lian Guo, Wenjun Liu, Teng Zhang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Apoptosis Tretinoin Biology Resting Phase Cell Cycle Biochemistry Flow cytometry 03 medical and health sciences hemic and lymphatic diseases Genetics medicine Humans Molecular Biology Homeodomain Proteins medicine.diagnostic_test Gene Expression Regulation Leukemic Cell growth G1 Phase Myeloid leukemia Articles Cell cycle Neoplasm Proteins all-trans retinoic acid Leukemia Myeloid Acute 030104 developmental biology medicine.anatomical_structure Oncology Cell culture Cancer research Molecular Medicine homeobox A5 gene K562 Cells K562 cells |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2016.5086 |
| Popis: | Leukemia is the most common malignant disease in children with high incidence and mortality rates, and a poor treatment effect. The aim of the present study was to examine the changes in the expression of homeobox (Hox) A5 gene and its relationship with cell cycle and apoptosis through the intervention of human K562 myeloid leukemia cell line by all-trans retinoic acid (ATRA), to analyze the role of HOXA5 in the pathogenesis and development process of myeloid leukemia. The optimal concentration of ATRA to be used with K562 cells was determined using a cell counting kit‑8 (CCK‑8). After 24, 72 and 48 h following treatment of K562 cells with 10 µmol/l ATRA, cell cycle events and apoptosis were measured using flow cytometry. HOXA5 mRNA and protein expression in K562 cells was assessed by RT‑PCR and western blot analysis, and the relationship between HOXA5 expression and cell cycle and apoptosis was analyzed. The HOXA5 mRNA and protein expression levels were increased following treatment with ATRA in K562 cells. Apoptosis was increased significantly. The cell cycle was inhibited in G0/G1 phase. Cell proliferation was also inhibited. HOXA5 mRNA and protein expression rates positively correlated with cell apoptosis and the increased percentage and cell cycle of the G0/G1 phase. However, HOXA5 negatively correlated with the reduced percentage of S stage. In conclusion, the expression of HOXA5 in cells was increased following treatment with ATRA in K562 cells, in a time-dependent manner. Additionally, ATRA may inhibit the proliferation of K562 cells and promote apoptosis by upregulating the HOXA5 mRNA and protein expression. |
| Databáze: | OpenAIRE |
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