SpeedScreen: The 'Missing Link' between Genomics and Lead Discovery
| Autor: | Natalie Lehmann, Francois Le Goff, Ireos Filipuzzi, Hartmut Zehender, Lorenz M. Mayr |
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| Rok vydání: | 2004 |
| Předmět: |
0301 basic medicine
Spectrometry Mass Electrospray Ionization Computer science High-throughput screening Genomics Sensitivity and Specificity 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry 03 medical and health sciences Lead (geology) Technology Pharmaceutical Chromatography High Pressure Liquid Selection (genetic algorithm) Protocol (science) Equipment Design Models Theoretical Combinatorial chemistry 0104 chemical sciences 010404 medicinal & biomolecular chemistry 030104 developmental biology Lead Drug Design Chromatography Gel Molecular Medicine Biochemical engineering Protein Binding Biotechnology |
| Zdroj: | SLAS Discovery. 9:498-505 |
| ISSN: | 2472-5552 |
| DOI: | 10.1177/1087057104267605 |
| Popis: | SpeedScreen is a novel, label-free, in-solution, affinity-based selection methodology for high-throughput screening (HTS) developed at Novartis Pharma. The SpeedScreen protocol comprises in-solution affinity selection, followed by size exclusion chromatography in combination with microbore-liquid-chromatography/electrospray-ionization mass spectrometry (micro-LC/ESI-MS). The authors describe the basic concept behind assay development, HTS, and data analysis with the SpeedScreen technology. Advantages and limitations of SpeedScreen compared to alternative screening technologies are discussed, and an example is given from a SpeedScreen campaign applying this innovative affinity selection concept in HTS. |
| Databáze: | OpenAIRE |
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