Comparison of three qPCR-based commercial tests for detection of periodontal pathogens
| Autor: | Mirella Rijnen, Cees Valkenburg, Fridus van der Weijden |
|---|---|
| Přispěvatelé: | Periodontology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
DNA
Bacterial 0301 basic medicine medicine.medical_specialty Science Real-Time Polymerase Chain Reaction Microbiology Article 03 medical and health sciences 0302 clinical medicine Internal medicine Retrospective analysis Humans Medicine Degree of similarity Periodontitis Retrospective Studies Multidisciplinary Bacteria business.industry 030206 dentistry medicine.disease 030104 developmental biology Risk factors Reagent Kits Diagnostic business |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) Scientific Reports, 11(1):6141. Nature Publishing Group Van der Weijden, F, Rijnen, M & Valkenburg, C 2021, ' Comparison of three qPCR-based commercial tests for detection of periodontal pathogens ', Scientific Reports, vol. 11, no. 1, 6141 . https://doi.org/10.1038/s41598-021-85305-3 Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-85305-3 |
| Popis: | In periodontal practice microbial results of periodontal test kits for identification of key pathogens are an aid in the treatment planning. Information on the performance of commercially available test kits is therefore essential for the clinician. In this retrospective analysis three commercially available qPCR kits for detection and quantification of selected periodontal bacterial species were compared, using 100 clinical samples from patients with untreated periodontitis. The analysis involved two separate comparisons in which kit A (LabOral Diagnostics, The Netherlands) was compared with kit B (Advanced Dental Diagnostics, The Netherlands), and with kit C (OralDent diagnostics, The Netherlands). Analytic procedures for detection and quantification of selected periodontal bacterial species were carried out according to the instructions of the laboratories. Kit A detected target species more often, and absolute numbers of bacterial cells were higher than with kit B. A high degree of similarity was found between the test outcomes by kit A and kit C. All three kits performed satisfactory but small and significant differences exist between kits. |
| Databáze: | OpenAIRE |
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