Structural and functional characterization of tree proteins involved in redox regulation: a new frontier in forest science

Autor: Jean-Pierre Jacquot, Jérémy Couturier, Nicolas Rouhier, Mélanie Morel-Rouhier, Christophe Plomion, Eric Gelhaye, Desirée D. Gütle, Arnaud Hecker, Claude Didierjean
Přispěvatelé: Interactions Arbres-Microorganismes (IAM), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Cristallographie, Résonance Magnétique et Modélisations (CRM2), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Biodiversité, Gènes & Communautés (BioGeCo), Université de Bordeaux (UB)-Institut National de la Recherche Agronomique (INRA), Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg [Freiburg], Fakultät für Biologie = Faculty of Biology [Freiburg], Albert-Ludwigs-Universität Freiburg, Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Annals of Forest Science
Annals of Forest Science, Springer Verlag/EDP Sciences, 2016, 73 (1), pp.119-134. ⟨10.1007/s13595-014-0442-9⟩
Annals of Forest Science 1 (73), 119-134. (2016)
Annals of Forest Science, Springer Nature (since 2011)/EDP Science (until 2010), 2016, 73 (1), pp.119-134. ⟨10.1007/s13595-014-0442-9⟩
ISSN: 1286-4560
1297-966X
DOI: 10.1007/s13595-014-0442-9⟩
Popis: Key message This paper describes how the combination of genomics, genetic engineering, and 3D structural characterization has helped clarify the redox regulatory networks in poplar with consequences not only in system biology in plants but also in bacteria and mammalian systems.[br/] Context Tree genomes are increasingly available with a large number of orphan genes coding for proteins, the function of which is still unknown.[br/] Aims and methods Modern techniques of genome analysis coupled with recombinant protein technology and massive 3D structural determination of tree proteins should help elucidate the function of many of the proteins encoded by orphan genes. X-ray crystallography and NMR will be the methods of choice for protein structure determination.[br/] Results In this review, we provide examples illustrating how the above-mentioned techniques improved our understanding of redox regulatory circuits in poplar, the first forest tree species sequenced. We showed that poplar peroxiredoxins use either thioredoxin or glutaredoxin as electron donors to reduce hydrogen peroxide. That glutaredoxin could be a reductant was unknown at the time of this discovery even in other biological organisms and was later confirmed notably by the observation that the two genes are fused in some bacteria and by the resolution of the structure of the bacterial hybrid protein. Similarly, genome analysis coupled to in vitro analysis of enzymatic properties led to the discovery that some plant methionine sulfoxide reductases can also use both thioredoxins and glutaredoxins as electron donors. Besides their disulfide reductase activity, it has been demonstrated that some poplar glutaredoxins are also involved in iron-sulfur center biogenesis and assembly. The original 3D structure determination has been made with poplar glutaredoxin C1 and then confirmed in a variety of other biological organisms including human. Our work also showed that in plants, so-called glutathione peroxidases use thioredoxins and not glutathione as electron donors. This is true for all non-selenocysteine-containing glutathione peroxidases. Finally, connections between the thioredoxin and glutaredoxin systems have been elucidated through the study of atypical poplar thioredoxins.[br/] Conclusion Altogether, these data illustrate how the combination of genetic engineering and structural biology improves our understanding of biological processes and helps fuel systems biology for trees and other biological species.
Databáze: OpenAIRE
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