Use of a PCR Assay for the Specific and Sensitive Detection of Trypanosoma Spp. in Naturally Infected Dairy Cattle in Peri-urban Kampala, Ugandaa
| Autor: | A. Wiemann, RJ Patzelt, A.S. Peregrine, D. Kakaire, D. Mehlitz, CJ Poetzsch, Peter-Henning Clausen |
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| Rok vydání: | 1998 |
| Předmět: |
Trypanosoma
Veterinary medicine Trypanosoma brucei brucei Pcr assay Cattle Diseases Hematocrit Trypanosoma brucei Polymerase Chain Reaction Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology law.invention History and Philosophy of Science Trypanosomiasis law parasitic diseases Prevalence medicine Animals Uganda Trypanosoma vivax Polymerase chain reaction Dairy cattle DNA Primers biology medicine.diagnostic_test General Neuroscience Trypanosomiasis Bovine Urban Health DNA Protozoan biology.organism_classification medicine.disease Virology Cross-Sectional Studies Trypanosomiasis African Cattle Female |
| Zdroj: | Annals of the New York Academy of Sciences. 849:21-31 |
| ISSN: | 0077-8923 |
| DOI: | 10.1111/j.1749-6632.1998.tb11029.x |
| Popis: | The objective of this study was to compare the sensitivity and specificity of the polymerase chain reaction (PCR) with the hematocrit centrifugation technique (HCT) and the mini-anion-exchange centrifugation technique (m-AECT) for diagnosis of trypanosome infections in livestock. In a cross-sectional study, 486 cattle from 50 randomly selected farms in Mukono County, Uganda were investigated in June 1994. The direct parasitological techniques were performed in the field, resulting in 45 (9.3%) animals positive by HCT and 78 (16%) positive by m-AECT. The total prevalence (combined evidence of HCT and m-AECT) was 18.9%, with 78.2% Trypanosoma brucei only, 10.9% T. vivax and 10.9% mixed (T. bruceil T. vivax) infections. Trypanosomes of the subgenus Nannomonas were not detected. DNA was prepared by lysis from 181 randomly selected blood samples and amplified by PCR using species-specific oligonucleotide primers. Overall, the PCR gave positive results in 63 (34.8%) blood samples, with 76.2% positive only for T. brucei, 20.6% positive only for T. vivax and 3.2% positive for mixed (T. bruceil T. vivax) infections. The preliminary results from this study demonstrate that the detection rate of PCR is about two times higher than that of the direct parasitological techniques, suggesting a higher sensitivity. The higher proportion of T. vivax infections detected by PCR as compared to HCT/m-AECT is likely to be due to false parasitological classifications which might occur under field conditions. |
| Databáze: | OpenAIRE |
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