Acetylcholine esterase is a regulator of GFAP expression and a target of dichlorvos in astrocytic differentiation of rat glioma C6 cells
Autor: | Erina Kadowaki, Kazuyoshi Arishima, Natsuko Hemmi, Takuya Kaneko, Shuntaro Hara, Yosuke Haga, Aisa Ozawa, Masako Yamamoto, Kou Sakabe, Takehiro Maki, Hitomi Sekiguchi, Motoharu Sakaue |
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Rok vydání: | 2013 |
Předmět: |
Neuropathy target esterase
chemistry.chemical_compound Astrocyte differentiation Fatty acid amide hydrolase Glial Fibrillary Acidic Protein Cyclic AMP Tumor Cells Cultured medicine Animals Cyclic adenosine monophosphate Molecular Biology Glial fibrillary acidic protein biology Paraoxon General Neuroscience Cell Differentiation Glioma Rats Cell biology medicine.anatomical_structure chemistry Biochemistry Astrocytes Dichlorvos Acetylcholinesterase biology.protein Neurology (clinical) Acetylcholine Developmental Biology Astrocyte medicine.drug |
Zdroj: | Brain Research. 1537:37-45 |
ISSN: | 0006-8993 |
Popis: | The main target of neurotoxins is neurons because they comprise the main part of neural function, but glial cells may be indirect targets because they support the function of neurons. Among the glial cells, astrocytes in particular act as "nurse cells", regulating neuronal survival and functions. In the present study, to reveal whether a known neurotoxic substance, organophosphate dichlorvos (DDVP), affects the differentiation of astrocytes, we used an astrocyte differentiation model in rat glioma C6 cells. Morphological change and induction of GFAP expression in the differentiating C6 cells were suppressed by DDVP treatment. The known potential targets of DDVP are acetylcholine esterase (AChE), fatty acid amide hydrolase and methyl guanine methyl transferase. Among the specific inhibitors against these enzymes, the AChE inhibitor paraoxon successfully suppressed the cellular morphological changes and the induction of GFAP expression in differentiating C6 cells. These results indicate that DDVP inhibits differentiation in the C6 astrocyte-differentiation model, in which at least AChE inhibition is involved and that AChE is a potent regulator of the differentiation. Furthermore, considering that the main substrate of AChE is ACh, thus, ACh may act as regulators of astrocyte differentiation. |
Databáze: | OpenAIRE |
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