Draxin-mediated Regulation of Granule Cell Progenitor Differentiation in the Postnatal Hippocampal Dentate Gyrus
| Autor: | Hideaki Tanaka, Ruhul Amin, Helen M. Cooper, Yuiko Morita-Fujimura, Yohei Shinmyo, Shuntaro Ikawa, Hirohisa Yamada, Hiroshi Tawarayama |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Neurogenesis Population Biology Hippocampal formation Hippocampus Subgranular zone Mice 03 medical and health sciences 0302 clinical medicine Neural Stem Cells medicine Animals Progenitor cell education Cell Proliferation education.field_of_study General Neuroscience Dentate gyrus Cell Differentiation Nestin Granule cell Cell biology 030104 developmental biology medicine.anatomical_structure Dentate Gyrus Intercellular Signaling Peptides and Proteins 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience. 431:184-192 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2020.02.005 |
| Popis: | The hippocampus is characterized by the presence of life-long neurogenesis. To elucidate the molecular mechanism regulating hippocampal neurogenesis, we studied the functions of the chemorepellent Draxin in neuronal proliferation and differentiation in the postnatal dentate gyrus. The present in vivo cell labeling and fate tracking analyses revealed enhanced differentiation of hippocampal neural stem and progenitor cells (hNSPCs) in the subgranular zone (SGZ) of Draxin-deficient mice. We observed a reduction in the number of BrdU-pulse labeled or Ki-67 immunopositive SGZ cells in the mutant mice. However, Draxin deficiency did not affect cell cycle duration of SGZ cells. In situ hybridization analysis indicated that the receptor component of the canonical Wnt pathway, Lrp6, is expressed in SGZ cells, including Nestin and Sox2 double-positive hNSPCs. Taken together with the previous finding that Draxin interacts physically with Lrp6, we postulate that Draxin plays a pivotal role in the regulation of Wnt-driven hNSPC differentiation to modulate the rate of neuronal differentiation in the progenitor population. |
| Databáze: | OpenAIRE |
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