Investigations of urothelial cells seeded on commercially available small intestine submucosa
| Autor: | Hans-Oliver Rennekampff, Ursula Kuehs, Jutta Krug, Arnulf Stenzl, S. Corvin, Sabine Maurer, Margot Christ-Adler, Karl-Dietrich Sievert, Gerhard Feil, Jörg Hennenlotter |
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| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Urothelial Cell business.industry Cell Survival Urology Cell Histology In Vitro Techniques Staining medicine.anatomical_structure Tissue engineering Cell culture Culture Media Conditioned Intestine Small medicine Cell Adhesion Cytotoxic T cell Humans Viability assay Intestinal Mucosa Urothelium business Cells Cultured |
| Zdroj: | European urology. 50(6) |
| ISSN: | 0302-2838 |
| Popis: | Objectives To examine adherence and viability of human urothelial cells seeded on commercially available small intestine submucosa (SIS ® ) specimens under serum-free conditions. Materials and methods Before seeding, SIS ® was either washed with incubation medium or coated with collagen A, fibronectin, or pronectin. A possible influence of SIS ® itself on the viability of urothelial cells was analysed with conditioned cell culture medium obtained by incubation of SIS ® for 24hours. In addition, untreated SIS ® and a setting without SIS ® were used as controls. Viability of urothelial cells was analysed with the WST-1 assay until day 9. Histology of seeded and unseeded SIS ® specimens was investigated after Papanicolaou staining. To demonstrate urothelial cell adherence on SIS ® , immunohistology was performed with a mixture of monoclonal AE1 and AE3 anticytokeratin antibodies. Results Urothelial cells seeded on SIS ® revealed no measurable cell viability. SIS ® -conditioned cell culture medium was cytotoxic for urothelial cells after 24hours. Histology only demonstrated cell nuclei and no cytoplasm both in seeded and unseeded SIS ® specimens, thus indicating porcine DNA. Expression of the cell type-specific marker proteins AE1/AE3 could not be demonstrated. Conclusion Since the commercially available SIS ® specimens used contained porcine DNA residues and demonstrated cytotoxic effects on urothelial cells, SIS ® is not suitable for in vitro construction of urothelial cell-matrix implants. |
| Databáze: | OpenAIRE |
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