Autoantibodies to the delta-opioid receptor function as opioid agonists and display immunomodulatory activity
| Autor: | Miranda K. Adelman, Parvathi Ranganathan, Samuel F. Schluter, Hao Chen |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Narcotics
medicine.medical_specialty Receptors CXCR4 Receptors CCR4 Time Factors medicine.drug_class Narcotic Antagonists Immunology CHO Cells Biology Transfection Article chemistry.chemical_compound Cricetulus Downregulation and upregulation Opioid receptor Internal medicine Cricetinae Receptors Opioid delta Gene expression medicine Cyclic AMP Immunology and Allergy Animals Humans Immunologic Factors Drug Interactions RNA Messenger Phosphorylation Regulation of gene expression Forskolin Blood Cells Colforsin Autoantibody Immunoglobulins Intravenous Flow Cytometry Endocrinology Neurology chemistry Opioid Gene Expression Regulation Ethanolamines Fatty Acids Unsaturated Neurology (clinical) medicine.drug Protein Binding |
| Zdroj: | Journal of neuroimmunology. 217(1-2) |
| ISSN: | 1872-8421 |
| Popis: | In this report, we show that affinity purified human anti-Delta Opioid Receptor (DOR) autoantibodies from IVIG are specific to DOR and possess agonistic properties displayed by their ability to dramatically decrease forskolin stimulated cAMP accumulation. Anti-DOR autoantibody also caused phosphorylation of the opioid receptor. Anti-DOR autoantibody treatment showed a significant reduction in CXCR4 gene expression as well as surface protein expression. In contrast, anti-DOR autoantibody treatment significantly upregulated CCR5 gene and protein expression. The presence of anti-DOR autoantibodies in IVIG and their potent immunomodulatory activity is further evidence to support the crosstalk between the neuroendocrine and immune system. |
| Databáze: | OpenAIRE |
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