Intra-articular Injection of Hyaluronan Diminishes Loss of Chondrocytes in a Rat Immobilized-Knee Model
| Autor: | Akira Ando, Eiji Itoi, Kouki Hatori, E. Chimoto, Y. Onoda, Yoshihiro Hagiwara |
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| Rok vydání: | 2008 |
| Předmět: |
Male
musculoskeletal diseases medicine.medical_specialty Pathology Knee Joint Adult male medicine.medical_treatment Articular cartilage Osteoarthritis Functional Laterality General Biochemistry Genetics and Molecular Biology Injections Intra-Articular Rats Sprague-Dawley Intra articular medicine Animals Femur Hyaluronic Acid Saline Joint immobilization Tibia business.industry General Medicine Anatomy musculoskeletal system medicine.disease Rats Saline group Hindlimb Suspension Orthopedic surgery business |
| Zdroj: | The Tohoku Journal of Experimental Medicine. 215:321-331 |
| ISSN: | 1349-3329 0040-8727 |
| DOI: | 10.1620/tjem.215.321 |
| Popis: | Joint immobilization is a useful and common treatment modality in orthopedics. However, it also causes unfavorable outcome such as articular cartilage degeneration. Intra-articular injection of hyaluronan has been accepted as a treatment of osteoarthritis, but its effects on immobilized joint remain to be clarified. Hyaluronan is a polysaccharide, distributed ubiquitously in various tissues. In this study, we examined the effect of hyaluronan on the articular cartilage in immobilized joints. The unilateral knee joints of adult male rats were immobilized at 150 degrees in flexion with an internal plate and screws for 1, 2, 4, 6, 8, 12, or 16 weeks (n = 84). Hyaluronan or saline (50 microl/each injection) was administered intra-articularly on the day of surgery and once a week. The articular cartilage from the medial midcondylar region of the knee was obtained, and divided into non-contact, contact and transitional areas (between the non-contact and the contact areas). In each area, a degree of degeneration was evaluated by histomorphometric grading, and measurements of thickness and number of chondrocytes. Histological grading scores in the hyaluronan group were smaller at 12 and 16 weeks compared with those in the saline group. The thickness of the articular cartilage increased in the transitional area in both groups. The number of chondrocytes in the contact and transitional areas gradually decreased, but their number in the hyaluronan group was greater at 12 and 16 weeks compared with that in the saline group. Hyaluronan showed chondroprotective effects on the articular cartilage in a rat immobilized-knee model. |
| Databáze: | OpenAIRE |
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