Brief exposure to cigarette smoke impairs airway epithelial cell innate anti-viral defence
| Autor: | Linping Chen, Kenneth Liu, Jayden Logan, Emmanuelle Fantino, Peter D. Sly, Catherine L. Gangell |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Rhinovirus
medicine.medical_treatment Bronchi Toxicology medicine.disease_cause Tobacco smoke Smoke Tobacco medicine CXCL10 Humans Respiratory system Cells Cultured Innate immune system business.industry Interleukin-8 Epithelial Cells General Medicine Interferon-beta Immunity Innate Chemokine CXCL10 Cytokine Viral replication Immunology business Intracellular |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 28(8) |
| ISSN: | 1879-3177 |
| Popis: | Human rhinovirus (hRV) infections commonly cause acute upper respiratory infections and asthma exacerbations. Environmental cigarette smoke exposure is associated with a significant increase in the risk for these infections in children.To determine the impact of short-term exposure to cigarette smoke on innate immune responses of airway epithelial cells infected with hRV.A human bronchial epithelial cell line (HBEC-3KT) was exposed to cigarette smoke extract (CSE) for 30 min and subsequently infected with hRV serotype 1B. Viral-induced cytokine release was measured with AlphaLISA and viral replication quantified by shed viral titer and intracellular viral copy number 24h post-infection.CSE induced a concentration-dependent decrease in CXCL10 (p0.001) and IFN-β (p0.001), with a 79% reduction at the highest dose with an associated 3-fold increase in shed virus. These effects were maintained when infection was delayed up to 24h post CSE exposure. Exogenous IFN-β treatment at t=0 after infection blunts the effects of CSE on viral replication (p0.05).A single exposure of 30 min to cigarette smoke has a lasting impact on epithelial innate defence providing a plausible mechanism for the increase in respiratory infections seen in children exposed to second-hand tobacco smoke. |
| Databáze: | OpenAIRE |
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