4Pi microscopy of type A with 1-photon excitation in biological fluorescence imaging
| Autor: | Johann Engelhardt, Marion C. Lang, Stefan W. Hell, Tobias Müller |
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| Rok vydání: | 2009 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science Optics Two-photon excitation microscopy Super-resolution microscopy business.industry Light sheet fluorescence microscopy Resolution (electron density) Microscopy Scanning confocal electron microscopy Photoactivated localization microscopy business Atomic and Molecular Physics and Optics |
| Zdroj: | Optics express. 15(5) |
| ISSN: | 1094-4087 |
| Popis: | We demonstrate that oil immersion lenses with a semi-aperture angle/= 74 degrees enable 4Pi confocal fluorescence microscopy of type A with 1 photon excitation. The axial sidelobes amount to50 % of the main diffraction maximum, implying that lobe induced artifacts can be removed from the image data. The advancement reported herein enables a relative inexpensive implementation of 4Pi microscopy, providing axially superresolved 3D-imaging in transparent samples. As an example, we show dual-color 4Pi images of double stained Golgi stacks in a mammalian cell with 110 nm axial resolution. The resolution can be further enhanced to values slightly below 100 nm by image deconvolution. |
| Databáze: | OpenAIRE |
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