HIV type-1 infection of the cotton rat (Sigmodon fulviventerandS. hispidus)
| Autor: | Harold S. Ginsberg, Raymond J. Langley, Gregory A. Prince |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
viruses
Sigmodon fulviventer HIV Infections Virus Replication medicine.disease_cause Virus Proviruses Species Specificity medicine Animals Humans Sigmodontinae Cotton rat Cells Cultured Multidisciplinary biology Reverse Transcriptase Polymerase Chain Reaction Viral culture Poliovirus Biological Sciences Provirus Sigmodon hispidus biology.organism_classification Genes gag Virology Viral replication DNA Viral HIV-1 Leukocytes Mononuclear RNA Viral Disease Susceptibility |
| Zdroj: | Proceedings of the National Academy of Sciences. 95:14355-14360 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.95.24.14355 |
| Popis: | Cotton rats (Sigmodon hispidusandS. fulviventer) are susceptible to many viruses that infect humans (e.g., poliovirus, respiratory syncytial virus, influenza virus, adenovirus, and parainfluenza virus) and have been influential in developing therapeutic clinical intervention strategies for many viral infections of man. This study set out to determine whether cotton rats are susceptible to infection with HIV type 1 (HIV-1). Results indicate that HIV-1 does infect the cotton rat andS. fulviventeris more susceptible thanS. hispidus. The virus was passaged from animal to animal for a total of three serial passages; but HIV replicated poorlyin vivo, was only detectable as proviral DNA, and never exceeded one provirus per 1.8 × 105cotton rat peripheral blood mononuclear cells. Infection induced a distinct and characteristic anti-HIV antibody response that, in some animals, included neutralizing antibodies, recognized all of the major HIV-1 antigens and the antibodies lasted out to 52 wk post-infection. NeonateS. fulviventerwere not more susceptible to infection than adults.In vitroculture studies produced indirect evidence of viral replication by detection of viral gag gene RNA in reverse transcriptase–PCR assays on viral culture supernatants. Collectively, these results indicate that HIV-1 can replicate in a nontransgenic rodent and that this system may have potential as an animal model for HIV-1 infection if viral replication rates can be improvedin vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|