Follow-up testing of borderline SARS-CoV-2 patients by rRT-PCR allows early diagnosis of COVID-19
| Autor: | Liesbeth Bruckers, K. Magerman, Petra Hilkens, Luc Waumans, Joost Boeckmans, Reinoud Cartuyvels, M. Raymaekers |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology rRT-PCR Real-Time Polymerase Chain Reaction Negative Test Result Article molecular diagnostics 03 medical and health sciences COVID-19 Testing 0302 clinical medicine Internal medicine medicine Humans 030212 general & internal medicine Positive test Retrospective Studies business.industry SARS-CoV-2 borderline COVID-19 Liter General Medicine Gold standard (test) Test (assessment) Reverse transcription polymerase chain reaction body regions Early Diagnosis Infectious Diseases business Follow-Up Studies |
| Zdroj: | Diagnostic Microbiology and Infectious Disease |
| ISSN: | 1879-0070 0732-8893 |
| Popis: | Detection of SARS-CoV-2 RNA in nasopharyngeal samples using the real-time reverse transcription polymerase chain reaction (rRT-PCR) is the gold standard for diagnosing COVID-19. Determination of SARS-CoV-2 RNA by rRT-PCR sometimes results in an inconclusive test result due to a high cycle threshold-value. We retrospectively analyzed 30,851 SARS-CoV-2 rRT-PCR test results. Borderline positivity was considered as the presence of ≤25 viral copies per milliliter, while no amplification was considered as a negative test result. Of all test results, 204 were answered as borderline, of which 107 were accompanied by a follow-up test within 96 hours. Of the 107 follow-up samples, 10 (9.35%) were found positive for SARS-CoV-2. COVID-19 symptoms were not predictive for testing positive in the follow-up test. The positive SARS-CoV-2 samples in the follow-up group represented 0.92% of all positive test results, highlighting the need for retesting and increased hygienic measures for borderline SARS-CoV-2 patients [NCT04636294]. |
| Databáze: | OpenAIRE |
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