Imiquimod Injection to Rabbit Vocal Folds: A Preliminary, Preclinical Investigation
Autor: | Stephanie Teng, Danielle N. Sin, Ryan C. Branski, Nao Hiwatashi, Gregory R. Dion, Peter A. Benedict, Milan R. Amin |
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Rok vydání: | 2017 |
Předmět: |
medicine.medical_specialty
medicine.medical_treatment Laryngoscopy Urology Imiquimod Vocal Cords Injections Intralesional Laryngeal Edema 03 medical and health sciences 0302 clinical medicine Animals Medicine Laryngeal Neoplasms Saline Papilloma medicine.diagnostic_test Respiratory distress business.industry Interferon-alpha Histology Disease Models Animal medicine.anatomical_structure Otorhinolaryngology 030220 oncology & carcinogenesis Vocal folds Aminoquinolines Surgery Rabbits Recurrent Respiratory Papillomatosis business Biomarkers 030215 immunology medicine.drug |
Zdroj: | Otolaryngology–Head and Neck Surgery. 156:702-705 |
ISSN: | 1097-6817 0194-5998 |
Popis: | Objective Given the recalcitrant nature of recurrent respiratory papillomatosis, targeted therapies to reduce disease burden are fundamental to improved patient care paradigms. We seek to demonstrate the safety of imiquimod injection into vocal fold mucosa by evaluating the degree of laryngeal edema, histopathologic changes to vocal fold structure, and serologic interferon α (IFNα) levels following injection. Study Design Preclinical. Setting Academic institution. Subjects and Methods Six New Zealand White rabbits underwent unilateral injection of 100 µg of sterile imiquimod (1 µg/µL), with 100 µL of normal saline injected into the contralateral vocal fold. Direct laryngoscopy was performed on days 3, 7, and 30 following injection. Larynges from 3 rabbits were harvested on postinjection day 7 for histologic analysis. The remaining 3 rabbit larynges were harvested on day 30. Serial serum samples were drawn for IFNα quantification via immunoassay. Results No signs of respiratory distress were observed at any point. Vocal fold appearance was not clinically divergent between imiquimod and control conditions via serial direct laryngoscopic evaluation. No inflammatory lesions or scarring were identified following injection. Histology showed no signs of acute inflammatory processes or changes in the control or imiquimod injection groups. Serum IFNα increased at days 3 and 7 following imiquimod injection ( P < .0001 and P = .0368, respectively), before returning to baseline by day 14. Conclusions Vocal fold imiquimod injection did not result in notable morbidity in this preclinical model. However, serum IFNα concentrations increased transiently. These data are critical to advance the therapeutic utility of this compound, particularly in the setting of recurrent respiratory papillomatosis. |
Databáze: | OpenAIRE |
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