Response‐Surface‐Optimized and Scaled‐Up Microbial Electrosynthesis of Chiral Alcohols
| Autor: | Natalia Klinger, Jeannine C. Mayr, Antje C. Spiess, Jan-Hendrik Grosch, Luis F. M. Rosa, Falk Harnisch |
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| Rok vydání: | 2020 |
| Předmět: |
microbial electrosynthesis
biocatalysis General Chemical Engineering 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences Electron transfer chemistry.chemical_compound Bioreactors enantioselectivity cofactors Escherichia coli medicine Environmental Chemistry General Materials Science Alcohol dehydrogenase Full Paper biology Lactobacillus brevis Alcohol Dehydrogenase Microbial electrosynthesis Full Papers 021001 nanoscience & nanotechnology biology.organism_classification Combinatorial chemistry 0104 chemical sciences Lactobacillus General Energy electrochemistry chemistry Biocatalysis Alcohols Yield (chemistry) biology.protein 0210 nano-technology NADP Acetophenone |
| Zdroj: | Chemsuschem |
| ISSN: | 1864-564X 1864-5631 |
| Popis: | A variety of enzymes can be easily incorporated and overexpressed within Escherichia coli cells by plasmids, making it an ideal chassis for bioelectrosynthesis. It has recently been demonstrated that microbial electrosynthesis (MES) of chiral alcohols is possible by using genetically modified E. coli with plasmid‐incorporated and overexpressed enzymes and methyl viologen as mediator for electron transfer. This model system, using NADPH‐dependent alcohol dehydrogenase from Lactobacillus brevis to convert acetophenone into (R)‐1‐phenylethanol, is assessed by using a design of experiment (DoE) approach. Process optimization is achieved with a 2.4‐fold increased yield of 94±7 %, a 3.9‐fold increased reaction rate of 324±67 μm h−1, and a coulombic efficiency of up to 68±7 %, while maintaining an excellent enantioselectivity of >99 %. Subsequent scale‐up to 1 L by using electrobioreactors under batch and fed‐batch conditions increases the titer of (R)‐1‐phenylethanol to 12.8±2.0 mm and paves the way to further develop E. coli into a universal chassis for MES in a standard biotechnological process environment. Powered up to implementation: Design of experiments and response surface methodology boosts the yields and efficiency of the enantioselective microbial electrosynthesis of (R)‐1‐phenylethanol by using resting Escherichia coli. Subsequent scale‐up to a 1 L electrobioreactor system, as well as fed‐batch operation, is a further important step towards commercial application. |
| Databáze: | OpenAIRE |
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