Assessment of urinary pharmacokinetics and pharmacodynamics of orbifloxacin in healthy dogs with ex vivo modelling
| Autor: | Takae Shimizu, Norihiko Ito, Kazuki Harada, Yoshiaki Hikasa, Saki Manabe, Taku Tsukamoto |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Staphylococcus Urinary system 030106 microbiology Microbial Sensitivity Tests Urine Pharmacology Microbiology Gas Chromatography-Mass Spectrometry 03 medical and health sciences Dogs Pharmacokinetics Ciprofloxacin Escherichia coli medicine Animals Urinary Tract Proteus mirabilis biology General Medicine biology.organism_classification Anti-Bacterial Agents Klebsiella pneumoniae Pharmacodynamics Pseudomonas aeruginosa Orbifloxacin Ex vivo medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 66:616-621 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Purpose. The aim of this study was to investigate the urinary pharmacokinetics (PK) of orbifloxacin (OBFX) administered at 5 mg kg−1 in six healthy dogs. A further aim was to use an ex vivo model to evaluate the urinary PK and pharmacodynamics (PD) of OBFX to determine its urinary bactericidal titre (UBT), which represents the maximal dilution of urine allowing bactericidal activity. Methodology. Fourteen urinary tract infection (UTI) pathogenic strains of five bacterial species (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis and Staphylococcus pseudintermedius) were used. Urine samples were obtained every 4 h for the first 24 h after OBFX administration, for measurement of urine drug concentration and UBT. Results/Key findings. The urine OBFX concentration peaked at 0–4, 4–8 or 4–8 h after administration, with a maximum concentration of 383±171 µg ml−1. Overall, the fluctuation in median UBT closely correlated with that of the mean urine OBFX concentration. In addition, the median areas under the UBT-time curves (AUBTs) were significantly inversely correlated with the MICs for OBFX in the tested strains (P |
| Databáze: | OpenAIRE |
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