Distinct triterpene synthases in the laticifers of Euphorbia lathyris
| Autor: | Irini Pateraki, Myriam Preiss, Albert Boronat, Emilio Centeno, Carmen Romero-Segura, Thomas J. Bach, Edith Forestier, Hubert Schaller, Sylvain Darnet, Vincent Compagnon, Anne Berna |
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| Přispěvatelé: | Agence Nationale de la Recherche (France), Ministerio de Ciencia e Innovación (España), Pateraki, Irene [0000-0002-7526-2334], Darnet, Sylvain [0000-0001-6351-0903], Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Universitat de Barcelona (UB), Federal University of Para - Universidade Federal do Para [Belem - Brésil], Pateraki, Irene, Darnet, Sylvain |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Latex lcsh:Medicine Cellular homeostasis Biology Article Terpene 03 medical and health sciences 0302 clinical medicine Triterpene Euphorbia Tobacco [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] lcsh:Science Intramolecular Transferases Enzyme Assays Plant Proteins 2. Zero hunger chemistry.chemical_classification Multidisciplinary ATP synthase Gene Expression Profiling lcsh:R [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics Plants Genetically Modified biology.organism_classification Triterpenes Yeast Plant Leaves 030104 developmental biology Enzyme Biochemistry chemistry Biofuels biology.protein lcsh:Q Heterologous expression 030217 neurology & neurosurgery |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Scientific Reports Scientific Reports, Nature Publishing Group, 2019, 9, pp.4840. ⟨10.1038/s41598-019-40905-y⟩ Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Euphorbia lathyris was proposed about fifty years ago as a potential agroenergetic crop. The tremendous amounts of triterpenes present in its latex has driven investigations for transforming this particular biological fluid into an industrial hydrocarbon source. The huge accumulation of terpenes in the latex of many plant species represent a challenging question regarding cellular homeostasis. In fact, the enzymes, the mechanisms and the controllers that tune the amount of products accumulated in specialized compartments (to fulfill ecological roles) or deposited at important sites (as essential factors) are not known. Here, we have isolated oxidosqualene cyclases highly expressed in the latex of Euphorbia lathyris. This triterpene biosynthetic machinery is made of distinct paralogous enzymes responsible for the massive accumulation of steroidal and non-steroidal tetracyclic triterpenes. More than eighty years after the isolation of butyrospermol from shea butter (Heilbronn IM, Moffet GL, and Spring FS J. Chem. Soc. 1934, 1583), a butyrospermol synthase is characterized in this work using yeast and in folia heterologous expression assays. This work was funded by a Plant Knowledge Based Bio Economy program ‘EULAFUEL’ (Plant KBBE 2009–2014) to Prof. Thomas J. Bach and Dr. Hubert Schaller (ANR09-KBBE-003-002), and to Prof. Albert Boronat (PLE2009-0003 from Ministerio de Ciencia e Innovación, Spain). |
| Databáze: | OpenAIRE |
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