Encapsulation of rotavirus into poly(lactide-co-glycolide) microspheres
Autor: | Ingrid Uhnoo, Lennart Svensson, Ali Mirazimi, Per Artursson, Kari Johansen, Johan Carlfors, Cecilia Sturesson, Raouf Ghaderi, Ann-Christine Albertsson |
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Rok vydání: | 1999 |
Předmět: |
Rotavirus
Chemistry Pharmaceutical Pharmaceutical Science Enzyme-Linked Immunosorbent Assay medicine.disease_cause Dosage form Mice In vivo Formaldehyde Polymer chemistry medicine Animals Antigens Particle Size Polyglactin 910 Active ingredient Mice Inbred BALB C Vaccines Chromatography Chemistry Aqueous two-phase system Microspheres Vortex Biodegradation Environmental Delayed-Action Preparations Immunoglobulin G Liberation Emulsions Female Drug carrier |
Zdroj: | Journal of Controlled Release. 59:377-389 |
ISSN: | 0168-3659 |
DOI: | 10.1016/s0168-3659(99)00014-0 |
Popis: | Two small-scale double emulsion techniques for incorporation of formaldehyde-inactivated rotavirus particles (FRRV) into poly(lactide-co-glycolide) (PLG) microspheres were developed and optimised. The effects of high-speed homogenisation versus vortex mixing on the double emulsion stability, microsphere size, entrapment efficiency and in vitro release of FRRV in the second emulsification step were studied. A stable double emulsion was verified only when using vortex mixing in this step. Slow removal of the organic phase allowed measurement of the size of the emulsion droplets and subsequent prediction of the size of the resulting microspheres. Microspheres in the size range of 1-10 microm were prepared using both techniques. The homogenisation technique was sensitive to changes in the operating time, the emulsification energy and the volume of the outer aqueous phase, while the vortex technique was more robust. Rotavirus was released in vitro in a triphasic manner with both techniques. The more robust vortex technique was selected for preparation of PLG microspheres containing rotavirus for in vivo studies. After immunisation of mice with a single intramuscular injection, the PLG-FRRV microspheres elicited an IgG antibody response in serum detected by ELISA equally high as that elicited with FRRV alone. These results indicate that the antigenicity of FFRV was retained after incorporation into PLG microspheres using the vortex technique. |
Databáze: | OpenAIRE |
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