Opposite effects of noradrenaline and acetylcholine upon hypocretin/orexin versus melanin concentrating hormone neurons in rat hypothalamic slices

Autor: Danièle Machard, Jeremy Grivel, Michel Muhlethaler, Laurence Bayer, Emmanuel Eggermann, Mauro Serafin, Barbara E. Jones
Rok vydání: 2004
Předmět:
Acetylcholine/pharmacology/ physiology
Hypothalamic Area
Lateral/cytology/drug effects/ metabolism
Patch-Clamp Techniques
Lateral hypothalamus
Neural Pathways/cytology/drug effects/metabolism
Action Potentials
Synaptic Transmission
Arousal/drug effects/physiology
Sleep/physiology
Rats
Sprague-Dawley
chemistry.chemical_compound
Norepinephrine
Neural Pathways
Pedunculopontine Tegmental Nucleus
Neurotransmitter
Neurons
Hypothalamic Hormones
General Neuroscience
Intracellular Signaling Peptides and Proteins
Intracellular Signaling Peptides and Proteins/ metabolism
Neurons/drug effects/ metabolism
Pedunculopontine Tegmental Nucleus/physiology
Neuropeptides/ metabolism
Pituitary Hormones/ metabolism
medicine.anatomical_structure
Locus Coeruleus
Arousal
hormones
hormone substitutes
and hormone antagonists
Acetylcholine
medicine.drug
medicine.medical_specialty
Models
Neurological
Neuropeptide
Neural Inhibition/drug effects/physiology
Hypothalamic Hormones/ metabolism
Biology
Cholinergic Agonists
Organ Culture Techniques
Norepinephrine/pharmacology/ physiology
Internal medicine
medicine
Animals
Melanins
Orexins
Neuropeptides
Neural Inhibition
Cholinergic Agonists/pharmacology
ddc:616.8
Orexin
Rats
Pituitary Hormones
Endocrinology
Action Potentials/drug effects/physiology
nervous system
chemistry
Hypothalamic Area
Lateral
Melanins/ metabolism
Synaptic Transmission/drug effects/physiology
Locus coeruleus
Cholinergic
Neuron
Sleep
Neuroscience
Locus Coeruleus/physiology
Zdroj: Neuroscience, Vol. 130, No 4 (2005) pp. 807-811
ISSN: 0306-4522
Popis: Hypocretin/orexin (Hcrt/Orx) and melanin concentrating hormone (MCH) are peptides contained in overlapping cell groups of the lateral hypothalamus and commonly involved in regulating sleep-wake states and energy balance, though likely in different ways. To see if these neurons are similarly or differentially modulated by neurotransmitters of the major brainstem arousal systems, the effects of noradrenaline (NA) and carbachol, a cholinergic agonist, were examined on identified Hcrt/Orx and MCH neurons in rat hypothalamic slices. Whereas both agonists depolarized and excited Hcrt/Orx neurons, they both hyperpolarized MCH neurons by direct postsynaptic actions. According to the activity profiles of the noradrenergic locus coeruleus and cholinergic pontomesencephalic neurons across the sleep-waking cycle, the Hcrt/Orx neurons would be excited by NA and acetylcholine (ACh) and thus active during arousal, whereas the MCH neurons would be inhibited by NA and ACh and thus inactive during arousal while disinhibited and possibly active during slow wave sleep. According to the present pharmacological results, Hcrt/Orx neurons may thus stimulate arousal in tandem with other arousal systems, whereas MCH neurons may function in opposition with other arousal systems and thus potentially dampen arousal to promote sleep.
Databáze: OpenAIRE
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