Pd–Ir Core–Shell Nanocubes: A Type of Highly Efficient and Versatile Peroxidase Mimic
| Autor: | Jiabin Liu, Kushal Ghale, Jingtuo Zhang, Ye Xu, Moon J. Kim, Xiaohu Xia, Haihang Ye, Erin McKenzie, Ning Lu |
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| Rok vydání: | 2015 |
| Předmět: |
General Physics and Astronomy
Enzyme-Linked Immunosorbent Assay Nanotechnology Iridium Horseradish peroxidase Catalysis Biomimetic Materials Limit of Detection medicine Humans General Materials Science Catalytic efficiency Versatile peroxidase Detection limit medicine.diagnostic_test biology Chemistry fungi General Engineering Prostate-Specific Antigen Combinatorial chemistry Peroxidases Nanocrystal Immunoassay biology.protein Nanoparticles Colorimetry Palladium Peroxidase |
| Zdroj: | ACS Nano. 9:9994-10004 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.5b03525 |
| Popis: | Peroxidase mimics with dimensions on the nanoscale have received great interest as emerging artificial enzymes for biomedicine and environmental protection. While a variety of peroxidase mimics have been actively developed recently, limited progress has been made toward improving their catalytic efficiency. In this study, we report a type of highly efficient peroxidase mimic that was engineered by depositing Ir atoms as ultrathin skins (a few atomic layers) on Pd nanocubes (i.e., Pd-Ir cubes). The Pd-Ir cubes exhibited significantly enhanced efficiency, with catalytic constants more than 20- and 400-fold higher than those of the initial Pd cubes and horseradish peroxidase (HRP), respectively. As a proof-of-concept demonstration, the Pd-Ir cubes were applied to the colorimetric enzyme-linked immunosorbent assay (ELISA) of human prostate surface antigen (PSA) with a detection limit of 0.67 pg/mL, which is ∼110-fold lower than that of the conventional HRP-based ELISA using the same set of antibodies and the same procedure. |
| Databáze: | OpenAIRE |
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