A 2,5-Dihydroxybenzoic Acid–Gelatin Conjugate Inhibits the Basal and Hsp90-Stimulated Migration and Invasion of Tumor Cells
| Autor: | Oleg S. Morenkov, Viktoria S. Petrenko, Alexey A. Leontievsky, Mariya A. Zhmurina, A. V. Lisov, Y. Y. Skarga, V. V. Vrublevskaya, Zoya A. Lisova, A. V. Snigireva |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
heparin-like polymer lcsh:Biotechnology Cell Biomedical Engineering Motility Article Biomaterials cell migration and invasion 03 medical and health sciences 0302 clinical medicine extracellular Hsp90 Heat shock protein lcsh:TP248.13-248.65 Extracellular medicine Fibrosarcoma 030304 developmental biology 0303 health sciences lcsh:R5-920 Heparin 2 5-DHBA–gelatin conjugate medicine.disease Cell biology 2 5-dihydroxybenzoic acid (2 5-DHBA) carbohydrates (lipids) medicine.anatomical_structure 030220 oncology & carcinogenesis HT1080 lcsh:Medicine (General) Conjugate medicine.drug |
| Zdroj: | Journal of Functional Biomaterials, Vol 11, Iss 39, p 39 (2020) Journal of Functional Biomaterials Volume 11 Issue 2 |
| ISSN: | 2079-4983 |
| Popis: | The extracellular cell surface-associated and soluble heat shock protein 90 (Hsp90) is known to participate in the migration and invasion of tumor cells. Earlier, we demonstrated that plasma membrane-associated heparan sulfate proteoglycans (HSPGs) bind the extracellular Hsp90 and thereby promote the Hsp90-mediated motility of tumor cells. Here, we showed that a conjugate of 2,5-dihydroxybenzoic acid with gelatin (2,5-DHBA&ndash gelatin), a synthetic polymer with heparin-like properties, suppressed the basal (unstimulated) migration and invasion of human glioblastoma A-172 and fibrosarcoma HT1080 cells, which was accompanied by the detachment of a fraction of Hsp90 from cell surface HSPGs. The polymeric conjugate also inhibited the migration/invasion of cells stimulated by exogenous soluble native Hsp90, which correlated with the inhibition of the attachment of soluble Hsp90 to cell surface HSPGs. The action of the 2,5-DHBA&ndash gelatin conjugate on the motility of A-172 and HT1080 cells was similar to that of heparin. The results demonstrate a potential of the 2,5-DHBA&ndash gelatin polymer for the development of antimetastatic drugs targeting cell motility and a possible role of extracellular Hsp90 in the suppression of the migration and invasion of tumor cells mediated by the 2,5-DHBA&ndash gelatin conjugate and heparin. |
| Databáze: | OpenAIRE |
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