Selective inhibition of Leishmania donovani by active extracts of wild mushrooms used by the tribal population of India: An in vitro exploration for new leads against parasitic protozoans
| Autor: | Debarati Mukherjee, Joydip Ghosh, Chiranjib Pal, Sirin Salma Sultana, Soumitra Paloi, Supratim Mandal, Somanjana Khatua, Aritri Dutta, Suvadip Mallick, Somaditya Dey, Krishnendu Acharya |
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| Rok vydání: | 2014 |
| Předmět: |
Immunology
Population Leishmania donovani India Apoptosis Complex Mixtures Nitric Oxide Inhibitory Concentration 50 Mice Lipid biosynthesis Animals education Amastigote Mice Inbred BALB C Mushroom education.field_of_study biology Astraeus hygrometricus Tricholoma Termitomyces Russula delica Dendritic Cells General Medicine biology.organism_classification Interleukin-12 Infectious Diseases Phytochemical Biochemistry Macrophages Peritoneal Parasitology Medicine Traditional Agaricales Hydrophobic and Hydrophilic Interactions Spleen |
| Zdroj: | Experimental Parasitology. 138:9-17 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2014.01.002 |
| Popis: | The study was intended at evaluating the anti-proliferating effect of mushrooms used in traditional folklore of Santal tribal population in India against Leishmania donovani (MHOM/IN/83/AG83). A total of eighteen extracts, three estracts from each mushroom [(80% ethanol extracted; Fa), (water-soluble polysaccharide fraction; Fb), (polyphenolic fraction; Fc)], from six wild mushrooms were obtained. These extracts were tested against the promastigotes and amastigotes for their antileishmanial capacity. Fa fractions (250 μg/mL) of Astraeus hygrometricus and Tricholoma giganteum significantly inhibited the growth of L. donovani promastigotes and interfered in lipid biosynthesis. Moreover, both fractions induced apoptosis in promastigotes. Water soluble Fb fractions of A. hygrometricus, Russula laurocerasi, Russula albonigra, Termitomyces eurhizus, Russula delica and polyphenolic Fc fraction of R. laurocerasi were found to inhibit the replication of intracellular amastigotes in macrophages dose dependently. Significantly, 50% inhibitory concentration of the active extracts against intracellular amastigotes induced release of nitric oxide and IL-12 in murine macrophages and dendritic cells assay and also found considerably non-toxic on murine splenocytes. Results of this study can be used as a basis for further phytochemical and pharmacological investigations in the effort for search of novel anti-leishmanial leads. |
| Databáze: | OpenAIRE |
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