Quantification of hair cortisol concentration in common marmosets (Callithrix jacchus) and tufted capuchins (Cebus apella)
| Autor: | Kathleen M. Brasky, Kimberley A. Phillips, Jerrold S. Meyer, Alyson N. Tukan, Anna D. Rigodanzo, Ryan T. Reusch |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine endocrine system Hydrocortisone Physiology Article 03 medical and health sciences 0302 clinical medicine biology.animal medicine Animals Cebus Ecology Evolution Behavior and Systematics integumentary system biology Animal health Marmoset Callithrix Upper thigh biology.organism_classification 030104 developmental biology medicine.anatomical_structure Female Animal Science and Zoology Neuroscience research hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery Hypothalamic–pituitary–adrenal axis Hair |
| Zdroj: | American Journal of Primatology. 80:e22879 |
| ISSN: | 0275-2565 |
| DOI: | 10.1002/ajp.22879 |
| Popis: | Quantifying cortisol concentration in hair is a non-invasive biomarker of long-term hypothalamic-pituitary-adrenal (HPA) activation, and thus can provide important information on laboratory animal health. Marmosets (Callithrix jacchus) and capuchins (Cebus apella) are New World primates increasingly used in biomedical and neuroscience research, yet published hair cortisol concentrations for these species are limited. Review of the existing published hair cortisol values from marmosets reveals highly discrepant values and the use of variable techniques for hair collection, processing, and cortisol extraction. In this investigation we utilized a well-established, standardized protocol to extract and quantify cortisol from marmoset (n = 12) and capuchin (n = 4) hair. Shaved hair samples were collected from the upper thigh during scheduled exams and analyzed via methanol extraction and enzyme immunoassay. In marmosets, hair cortisol concentration ranged from 2,710 to 6,267 pg/mg and averaged 4,070 ± 304 pg/mg. In capuchins, hair cortisol concentration ranged from 621 to 2,089 pg/mg and averaged 1,092 ± 338 pg/mg. Hair cortisol concentration was significantly different between marmosets and capuchins, with marmosets having higher concentrations than capuchins. The incorporation of hair cortisol analysis into research protocols provides a non-invasive measure of HPA axis activity over time, which offers insight into animal health. Utilization of standard protocols across laboratories is essential to obtaining valid measurements and allowing for valuable future cross-species comparisons. |
| Databáze: | OpenAIRE |
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