Polar Bear Encephalitis: Establishment of a Comprehensive Next-generation Pathogen Analysis Pipeline for Captive and Free-living Wildlife

Autor:	Wei Sun, Claudia A. Szentiks, R. Höveler, Peter Wohlsein, Martin Beer, A. Schüle, Veljko M. Nikolin, Armando Damiani, Mark D. Stenglein, Achim D. Gruber, Wei Chen, Matthias Scheuch, Marion L. East, Dirk Höper, Donata Kalthoff, Joseph L. DeRisi, Heribert Hofer, Kyriakos Tsangaras, Camila J. Mazzoni, Björn Abendroth, Gábor Á. Czirják, Annekatrin Aue, Felix Heeger, Alex D. Greenwood, Mirjam Grobbel, Nikolaus Osterrieder
Rok vydání:	2014
Předmět:	Diagnostic methods General Veterinary Animals zoo Ecology Ursus maritimus Wildlife Animals Wild Biology medicine.disease Diagnostic tools Pipeline (software) Pathology and Forensic Medicine Evolutionary biology biology.animal medicine Animals Encephalitis Animals Zoo Pathogen Ursidae
Zdroj:	Journal of Comparative Pathology; Vol 150 Journal of Comparative Pathology
ISSN:	0021-9975
Popis:	This report describes three possibly related incidences of encephalitis, two of them lethal, in captive polar bears (Ursus maritimus). Standard diagnostic methods failed to identify pathogens in any of these cases. A comprehensive, three-stage diagnostic 'pipeline' employing both standard serological methods and new DNA microarray and next generation sequencing-based diagnostics was developed, in part as a consequence of this initial failure. This pipeline approach illustrates the strengths, weaknesses and limitations of these tools in determining pathogen caused deaths in non-model organisms such as wildlife species and why the use of a limited number of diagnostic tools may fail to uncover important wildlife pathogens.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56b29e4080bcdfa9add8c83a006a0740 https://doi.org/10.1016/j.jcpa.2013.12.005 Zobrazit plný text záznamu Full Text from ScienceDirect