| Autor: |
Denver T. Niles, Rose A. Lee, Gabriella S. Lamb, Fatima Al Dhaheri, Juri Boguniewicz |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Diagnostic microbiology and infectious disease. 105(1) |
| ISSN: |
1879-0070 |
| Popis: |
Plasma cell-free metagenomic next-generation sequencing (cf-mNGS) is a non-invasive method that may be able to identify thousands of pathogens through a hypothesis-free approach. There is a lack of consensus on how this test compares to conventional microbiologic testing. We conducted a systematic review and meta-analysis of published studies evaluating the accuracy of plasma cf-mNGS in hospitalized patients and present pooled estimates of the positive (PPA) and negative percent agreement (NPA) compared to a composite reference standard that included all conventional microbiological testing and clinical history as assessed by an adjudication panel or clinical treatment team. Five retrospective studies (n = 552) were included. The majority of the patients (56%-88%) were immunocompromised. The pooled PPA was 67% (95% CI, 54%-81%) and the pooled NPA was 70% (95% CI, 63%-77%). The pooled diagnostic performance characteristics suggest that cf-mNGS provides limited evidence for ruling in or out the presence of infection as commonly used. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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