UHPLC-MS/MS method for iohexol determination in human EDTA and lithium-heparin plasma, human urine and in goat- and pig EDTA plasma
| Autor: | Maaike K. van Gelder, J Roggeveld, Daan J Touw, Ron T. Gansevoort, Jasper Stevens, Claire C J Dekkers, Remco A. Koster, Mireille A. Wessels, Hiddo J. Lambers Heerspink |
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| Přispěvatelé: | Groningen Kidney Center (GKC), Cardiovascular Centre (CVC), Real World Studies in PharmacoEpidemiology, -Genetics, -Economics and -Therapy (PEGET), Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Groningen Research Institute for Asthma and COPD (GRIAC), Critical care, Anesthesiology, Peri-operative and Emergency medicine (CAPE), Pharmaceutical Analysis, Medicinal Chemistry and Bioanalysis (MCB) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Bioanalysis
Swine Iohexol Clinical Biochemistry 030232 urology & nephrology Renal function Urine Lithium 01 natural sciences Edta plasma Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Pharmacokinetics Tandem Mass Spectrometry Heparin plasma medicine Animals Humans General Pharmacology Toxicology and Pharmaceutics Quadrupole mass analyzer Chromatography High Pressure Liquid Edetic Acid Chromatography Chemistry Heparin Goats 010401 analytical chemistry General Medicine 0104 chemical sciences Medical Laboratory Technology medicine.drug |
| Zdroj: | Bioanalysis, 12(14), 981-990. FUTURE SCI LTD |
| ISSN: | 1757-6180 |
| Popis: | Aim: Iohexol plasma clearance is used as an indicator of kidney function in clinical and preclinical settings. To investigate the pharmacokinetic profile of iohexol, a rapid, simple method for measurement of iohexol in different matrices and species was needed. Materials & methods: Iohexol was separated on an Accucore C18 column (Thermo Fisher Scientific, CA, USA). Detection was performed on a Thermo Scientific Quantiva tandem quadrupole mass spectrometer. The method was validated according to the requirements for bioanalytical methods issued by the US FDA and European Medicines Agency. Conclusion: We developed and validated a fast and efficient analytical method, suitable for analyzing iohexol in human EDTA plasma, human lithium-heparin plasma, human urine and goat- and pig EDTA plasma, using only one calibration line prepared in human EDTA plasma. |
| Databáze: | OpenAIRE |
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