Use of a multiplex PCR/RT-PCR approach to assess the viral causes of influenza-like illnesses in Cambodia during three consecutive dry seasons
Autor: | Carole Buecher, Wei Wang, Vincent Deubel, Veasna Duong, Astrid Vabret, François Freymuth, Sirenda Vong, Sek Mardy, Philippe Buchy, Monica Naughtin |
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Přispěvatelé: | Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS), Laboratoire de Virologie Humaine et Moléculaire [Caen], CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Wei Wang is a recipient of a fellowship allocated by AREVA, Buecher C, Mardy S, Wang W, Duong V, Vong S, Naughtin M, Vabret A, Freymuth F, Deubel V, Buchy P. |
Rok vydání: | 2010 |
Předmět: |
Adult
Male Adolescent viruses Respiratory Tract Diseases Biology medicine.disease_cause Asymptomatic Virus Article 03 medical and health sciences Young Adult 0302 clinical medicine Virology Multiplex polymerase chain reaction Influenza A virus medicine Prevalence asymptomatic individuals Humans 030212 general & internal medicine Preschool Child Aged 0303 health sciences Influenza-like illness influenza‐like illness 030306 microbiology Reverse Transcriptase Polymerase Chain Reaction virus diseases Infant Middle Aged multiplex PCR 3. Good health respiratory virus Infectious Diseases Real-time polymerase chain reaction Virus Diseases Child Preschool [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Viruses Respiratory virus Female Rhinovirus medicine.symptom Cambodia Research Article |
Zdroj: | Journal of Medical Virology Journal of Medical Virology, Wiley-Blackwell, 2010, 82 (10), pp.1762-72. ⟨10.1002/jmv.21891⟩ |
ISSN: | 1096-9071 0146-6615 |
Popis: | Acute respiratory infections are a major cause of mortality and morbidity worldwide. Using multiplex PCR/RT‐PCR methods for the detection of 18 respiratory viruses, the circulation of those viruses during 3 consecutive dry seasons in Cambodia was described. Among 234 patients who presented with influenza‐like illness, 35.5% were positive for at least one virus. Rhinoviruses (43.4%), parainfluenza (31.3%) viruses and coronaviruses (21.7%) were the most frequently detected viruses. Influenza A virus, parainfluenza virus 4 and SARS‐coronavirus were not detected during the study period. Ninety apparently healthy individuals were included as controls and 10% of these samples tested positive for one or more respiratory viruses. No significant differences were observed in frequency and in virus copy numbers for rhinovirus detection between symptomatic and asymptomatic groups. This study raises questions about the significance of the detection of some respiratory viruses, especially using highly sensitive methods, given their presence in apparently healthy individuals. The link between the presence of the virus and the origin of the illness is therefore unclear. J. Med. Virol. 82:1762–1772, 2010. © 2010 Wiley‐Liss, Inc. |
Databáze: | OpenAIRE |
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