Use of a multiplex PCR/RT-PCR approach to assess the viral causes of influenza-like illnesses in Cambodia during three consecutive dry seasons

Autor: Carole Buecher, Wei Wang, Vincent Deubel, Veasna Duong, Astrid Vabret, François Freymuth, Sirenda Vong, Sek Mardy, Philippe Buchy, Monica Naughtin
Přispěvatelé: Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS), Laboratoire de Virologie Humaine et Moléculaire [Caen], CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Wei Wang is a recipient of a fellowship allocated by AREVA, Buecher C, Mardy S, Wang W, Duong V, Vong S, Naughtin M, Vabret A, Freymuth F, Deubel V, Buchy P.
Rok vydání: 2010
Předmět:
Adult
Male
Adolescent
viruses
Respiratory Tract Diseases
Biology
medicine.disease_cause
Asymptomatic
Virus
Article
03 medical and health sciences
Young Adult
0302 clinical medicine
Virology
Multiplex polymerase chain reaction
Influenza A virus
medicine
Prevalence
asymptomatic individuals
Humans
030212 general & internal medicine
Preschool
Child
Aged
0303 health sciences
Influenza-like illness
influenza‐like illness
030306 microbiology
Reverse Transcriptase Polymerase Chain Reaction
virus diseases
Infant
Middle Aged
multiplex PCR
3. Good health
respiratory virus
Infectious Diseases
Real-time polymerase chain reaction
Virus Diseases
Child
Preschool
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Viruses
Respiratory virus
Female
Rhinovirus
medicine.symptom
Cambodia
Research Article
Zdroj: Journal of Medical Virology
Journal of Medical Virology, Wiley-Blackwell, 2010, 82 (10), pp.1762-72. ⟨10.1002/jmv.21891⟩
ISSN: 1096-9071
0146-6615
Popis: Acute respiratory infections are a major cause of mortality and morbidity worldwide. Using multiplex PCR/RT‐PCR methods for the detection of 18 respiratory viruses, the circulation of those viruses during 3 consecutive dry seasons in Cambodia was described. Among 234 patients who presented with influenza‐like illness, 35.5% were positive for at least one virus. Rhinoviruses (43.4%), parainfluenza (31.3%) viruses and coronaviruses (21.7%) were the most frequently detected viruses. Influenza A virus, parainfluenza virus 4 and SARS‐coronavirus were not detected during the study period. Ninety apparently healthy individuals were included as controls and 10% of these samples tested positive for one or more respiratory viruses. No significant differences were observed in frequency and in virus copy numbers for rhinovirus detection between symptomatic and asymptomatic groups. This study raises questions about the significance of the detection of some respiratory viruses, especially using highly sensitive methods, given their presence in apparently healthy individuals. The link between the presence of the virus and the origin of the illness is therefore unclear. J. Med. Virol. 82:1762–1772, 2010. © 2010 Wiley‐Liss, Inc.
Databáze: OpenAIRE
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