Green Transfection: Cationic Lipid Nanocarrier System Derivatized from Vegetable Fat, Palmstearin Enhances Nucleic Acid Transfections
| Autor: | Mohankumar K. Murugesan, Srujan Marepally, Arabinda Chaudhuri, Priya Dharmalingam, Rajkumar Banerjee, Saravanabhavan Thangavel, Bhanuprasad Bandlamudi, Chandrashekhar Voshavar, Hari Krishna R. Rachamalla, Brijesh Lohchania |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
010405 organic chemistry General Chemical Engineering Genetic enhancement Cationic polymerization General Chemistry Transfection Biology 01 natural sciences Article 0104 chemical sciences Green fluorescent protein Palmitic acid lcsh:Chemistry stomatognathic diseases 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biochemistry chemistry lcsh:QD1-999 Lipofectamine Nucleic acid Nanocarriers |
| Zdroj: | ACS Omega, Vol 2, Iss 11, Pp 7892-7903 (2017) ACS Omega |
| ISSN: | 2470-1343 |
| Popis: | Cationic lipid-guided nucleic acid delivery holds great promise in gene therapy and genome-editing applications for treating genetic diseases. However, the major challenge lies in achieving therapeutically relevant efficiencies. Prior findings, including our own, demonstrated that asymmetry in the hydrophobic core of cationic lipids imparted superior transfection efficiencies. To this end, we have developed a lipid nanocarrier system with an asymmetric hydrophobic core (PS-Lips) derived from a mixture of fatty acids of food-grade palmstearin and compared its efficiency with symmetric palmitic acid-based nanocarrier system (P-Lip). PS-Lips exhibited superior transfection efficiencies with both plasmid DNA (pDNA) and mRNA in multiple cultured cells than the control P-Lip. More importantly, PS-Lips exhibited 2-fold superior transfections with linear nucleic acid, green fluorescent protein (GFP) mRNA in hematopoietic cells, when compared with the commercial control lipofectamine RNAiMAX. PS-Lips was also found to be effective in delivering genome-editing tools (CRISPR/Cas9, sgRNA encoded pDNA with a reporter GFP construct) than P-Lip in HEK-293 cells. In the present study, we report that cationic liposomes derivatized from natural food-grade fat palmstearin with a natural hydrophobic core asymmetry are efficient in delivering both linear and circular nucleic acids. In particular, PS-Lips is efficient in delivering mRNA to hematopoietic cells. These findings can be further exploited in the genome-editing approach for treating β-globinopathies. |
| Databáze: | OpenAIRE |
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